scatter diagram showing peripheral blood lymphocytes stained with SF00204. The cells were incubated with the antibody (SF00204, 5 µl/Test) for 30 min at 22°C. Acquisition of >10,000 events was performed.
Blank control:Molt4.
Primary Antibody (green line): Mouse Anti-CD45 antibody (SF00204)
Dilution: 5μl /10^6 cells;
Isotype Control Antibody (orange line): Mouse IgG .
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.