Mouse Anti-Myc Tag antibody

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avian myelocytomatosis viral oncogene homolog; bHLHe39; c-Myc; class E basic helix-loop-helix protein 39; MRTL; MYC; Myc Epitope Tag; myc proto-oncogene protein; myc-related translation/localization regulatory factor; oncogene c-Myc; proto-oncogene c-Myc;

Cat:

SLM51003M

Species Reactivity：

Recombinant protein

Immunogen：

KLH conjugated synthetic peptide derived from human Myc Tag

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Monoclonal

Isotype：

IgG1

Applications：

WB=1:500-2000not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Mouse

Product Overview：

Sample: Lane1: Myc-Tagged Fusion Protein Overexpression E.coli Lysate at 2ug Lane2: Myc-Tagged Fusion Protein Overexpression E.coli Lysate at 2ug Primary: Anti-Myc tag (SLM51003M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 41 kD Observed band size: 45 kD Western blot analysis of 12-tag protein lysate, using Myc Tag Antibody(Cat. SLM51003M). SLM51003M was diluted at 1:2000. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysate at 20ug.Anti-Myc Tag Antibody at 1:2000 dilution + 12 tag recombinant protein lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 45-50 kDa

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Unit:

Price: $316.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties. The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast,insect and mammalian cell systems.
The c-Myc protein is a transcription factor, which is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin dependent kinase, ERK2 and SLCJun N terminal Kinase (JNK) in cell proliferation and cell cycle regulation. Studies also have shown that c-Myc is essential for tumor cell development in vasculogenesis and angiogenesis that distribute blood throughout the cells, and which brought extensive attention in the development of new therapeutic approach for cancer treatment.

SWISS:
P01106

Gene ID:
929

Database links:

Entrez Gene: 929 Human

Entrez Gene: 17869 Mouse

Entrez Gene: 24577 Rat

Omim: 190080 Human

SwissProt: P01106 Human

SwissProt: P01108 Mouse

SwissProt: P09416 Rat

Unigene: 202453 Human

Unigene: 2444 Mouse

Unigene: 12072 Rat

Picture

Western blot analysis of 12-tag protein lysate, using Myc Tag Antibody(Cat. SLM51003M). SLM51003M was diluted at 1:2000. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysate at 20ug.

Anti-Myc Tag Antibody at 1:2000 dilution + 12 tag recombinant protein lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 45-50 kDa

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