background:
AT-motif binding factor 1 (ATSF1) binds to the AT-rich core sequence element in the human a-fetoprotein enhancer (1). Alternative splicing generates the ATSF1-A and ATSF1-B (2,3). While ATSF1-A contains a 920-amino acid extension at the N-terminus, both ATSF1-A and ATSF1-B contain 4 DNA-binding homeobox domains (2,3). Additionally, ATSF1-A contains 23 zinc finger motifs while ATSF1-B contains 18 zinc finger motifs (1–3). The N-terminal extension unique to ATSF1-A has transcriptional repressor activity (4). In the small intestine, ATSF1-A inhibits expression of the brushborder enzyme aminopeptidase-N through direct binding to the AT motif element (5). Besides functioning in transcription regulation, ATSF1 also functions in ATPase activity (6). ATPase activity associated with ATSF1-A is DNA/RNA-dependent and requires both homeobox domains and zinc finger motifs (6). ATSF1 is highly expressed in spleen and brain tissues (7). The gene encoding human ATSF1 maps to chromosome 16q22.3-q23.1 (8).
Function:
ATSF1 is a transcription factor that negatively regulates alpha fetoprotein and MYB, transactivates CDKN1A, and may be a tumor suppressor. Loss of ATSF1 is one mechanism that defines the absence of growth control in prostate cancer. Presence of the isoform ABTF1-A is correlated with better prognosis in breast cancer and may also serve as a marker of endocrine responsiveness.
Subunit:
Interacts with FNBP3 (By similarity). Interacts with PIAS3.
Subcellular Location:
Nuclear
Tissue Specificity:
Not found in normal gastric mucosa but found in gastric carcinoma cells (at protein level).
Post-translational modifications:
Phosphorylated upon DNA damage, probably by ATM or ATR.
Similarity:
Contains 22 C2H2-type zinc fingers.
Contains 4 homeobox DNA-binding domains.
Database links:
UniProtKB/Swiss-Prot: Q15911.2
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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