KLH conjugated synthetic peptide derived from human CD4
Format：
Lyophilized or Liquid
Storage instructions：
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of ant
Buffer：
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Concentration：
1mg/ml
Clonality：
Monoclonal
Isotype：
IgG
Applications：
ICC=1:50-200IF=1:50-200not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Mouse
Calculated MW：
48kDa
Product Overview：
scatter diagram showing peripheral blood lymphocytes stained with SLM30093M-FITC. The cells were incubated with the antibody (SLM30093M-FITC, 5 µl/Test) for 30 min at 22°C. Acquisition of >10,000 events was performed.
background: This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Aug 2010].
Function: Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
Subunit: Associates with LCK. Binds to HISLV1 gp120 and to P4HB/PDI and upon HISLV1 binding to the cell membrane, is part of P4HB/PDI-CD4-CXCR4-gp120 complex. Interacts with HISLV1 Envelope polyprotein gp160 and protein Vpu. Interacts with Human Herpes virus 7 capsid proteins. Interacts with PTK2/FAK1; this interaction requires the presence of HISLV1 gp120.
Subcellular Location: Cell membrane; Single-pass type I membrane protein. Note=Localizes to lipid rafts. Removed from plasma membrane by HISLV1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HISLV1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
Post-translational modifications: Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Picture
scatter diagram showing peripheral blood lymphocytes stained with SLM30093M-FITC. The cells were incubated with the antibody (SLM30093M-FITC, 5 µl/Test) for 30 min at 22°C. Acquisition of >10,000 events was performed.
