Rabbit Anti-CLDN1 antibody | Sunlong Medical

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CLD1_HUMAN; Claudin-1; Claudin 1; Claudin1; CLD1; SEMP1; Senescence-associated epithelial membrane protein; UNQ481/PRO944; ILVASC;

Cat:

SL1428R

Species Reactivity：

Human,Mouse,Rat,

Immunogen：

KLH conjugated synthetic peptide derived from mouse Claudin 1:121-211/211<Cytoplasmic>

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Kidney (Mouse) Lysate at 40 ugPrimary: Anti-Claudin 1 (SL1428R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 23 kDObserved band size: 23 kDSample: Lane 1: A549 (Human) Cell Lysate at 30 ugLane 2: HepG2 (Human) Cell Lysate at 30 ugPrimary: Anti- Claudin 1 (SL1428R) at 1/1000 dilutionAnti- beta-Actin (SL0061R) at 1/2000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 21 kDObserved band size: 21 kDSample: HepG2(Human) Cell Lysate at 30 ugPrimary: Anti-Claudin 1 (SL1428R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 23 kDObserved band size: 23 kDSample: A431(Human) Cell Lysate at 30 ugPrimary: Anti-Claudin 1 (SL1428R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 23 kDObserved band size: 23 kDTissue/cell: rat intestine tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-Claudin-1 Polyclonal Antibody, Unconjugated(SL1428R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingBlank control: Raji(blue). Primary Antibody:Rabbit Anti-Claudin 1 antibody(SL1428R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.ProtocolThe cells were fixed with 2% paraformaldehyde (10 min). Antibody (SL1428R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody of SL1428R at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
Feedback Wall

Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Loss of function mutations result in neonatal ichthyosis-sclerosing cholangitis syndrome. [provided by RefSeq, Jul 2008]

Function:
Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.

Subunit:
Can form homo- and heteropolymers with other CLDN. Homopolymers interact with CLDN3, but not CLDN2, homopolymers. Directly interacts with TJP1/ZO-1, TJP2/ZO-2 and TJP3/ZO-3. Interacts with MPDZ and INADL.

Subcellular Location:
Cell junction, tight junction. Cell membrane; Multi-pass membrane protein.

Tissue Specificity:
Widely expressed, with highest levels in liver and kidney.

Similarity:
Belongs to the claudin family.

SWISS:
O88551

Gene ID:
12737

Database links:

Entrez Gene: 9076 Human

Entrez Gene: 12737 Mouse

Entrez Gene: 65129 Rat

SwissProt: O95832 Human

SwissProt: O88551 Mouse

SwissProt: P56745 Rat

Claudin 1蛋白是紧密连接区相关的转膜蛋白Claudin的家族成员，在构成紧密连接的完整膜蛋白中发挥着一定作用，目前多用于肿瘤方面的研究。 Picture

Sample:
Lane 1: A549 (Human) Cell Lysate at 30 ug
Lane 2: HepG2 (Human) Cell Lysate at 30 ug
Primary:
Anti- Claudin 1 (SL1428R) at 1/1000 dilution
Anti- beta-Actin (SL0061R) at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 21 kD
Observed band size: 21 kD

Sample:
HepG2(Human) Cell Lysate at 30 ug
Primary: Anti-Claudin 1 (SL1428R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 23 kD
Observed band size: 23 kD

Sample:
A431(Human) Cell Lysate at 30 ug
Primary: Anti-Claudin 1 (SL1428R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 23 kD
Observed band size: 23 kD

Tissue/cell: rat intestine tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-Claudin-1 Polyclonal Antibody, Unconjugated(SL1428R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Blank control: Raji(blue).
Primary Antibody:Rabbit Anti-Claudin 1 antibody(SL1428R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min). Antibody (SL1428R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody of SL1428R at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.

Product Feedback Wall

Specific References (10) | SL1428R has been referenced in 10 publications.

[IF=1.813] Zhang Boxun. et al. The Herbal Medicine Scutellaria-Coptis Alleviates Intestinal Mucosal Barrier Damage in Diabetic Rats by Inhibiting Inflammation and Modulating the Gut Microbiota. Evid-Based Compl Alt. 2020;2020:4568629 IHC ; Rat.

PubMed:33224253

[IF=7.561] Zhou Y. et al. The Panda-Derived Lactobacillus plantarum G201683 Alleviates the Inflammatory Response in DSS-Induced Panda Microbiota-Associated Mice.. Front Immunol. 2021 Dec;12:747045-747045 WB ; Mouse.

PubMed:3495636

[IF=3.052] Yilei Jing. et al. Matrine treatment induced an A2 astrocyte phenotype and protected the blood-brain barrier in CNS autoimmunity. J Chem Neuroanat. 2021 Nov;117:102004 WB ; Rat.

PubMed:34280490

[IF=3.289] Chen M et al. Dietary L-Tryptophan alleviated LPS-induced intestinal barrier injury by regulating tight junctions in Caco-2 cell monolayer model.Food Funct. 2019 May 22;10(5):2390-2398. WB ; Human.

PubMed:30977499

[IF=3.68] Wang, Tao, et al. "Notch inhibition promotes fetal liver stem/progenitor cells differentiation into hepatocytes via the inhibition of HNF-1β." Cell and Tissue Research (2014): 1-12. WB ; Rat.

PubMed:24737489

[IF=7.963] Jianzhao Liao. et al. Gut microbiota disturbance exaggerates battery wastewater-induced hepatotoxicity through a gut-liver axis. Sci Total Environ. 2022 Feb;809:152188 WB ; Mouse.

PubMed:34875328

[IF=3.531] Yanan Gao. et al. Aflatoxin B1 and Aflatoxin M1 Induce Compromised Intestinal Integrity through Clathrin-Mediated Endocytosis. Toxins. 2021 Mar;13(3):184 IF ; Mouse.

PubMed:33801329

[IF=3.943] Ning Xu. et al. Protective effect and mechanism of rebamipide on NSAIDs associated small bowel injury. Int Immunopharmacol. 2021 Jan;90:107136 WB ; Rat.

PubMed:33218942

[IF=2.959] Xu T et al. IL-1β induces increased tight junction permeability in bovine mammary epithelial cells via the IL-1β-ERK1/2-MLCK axis upon blood-milk barrier damage.J Cell Biochem. 2018 Nov;119(11):9028-9041. ICF&WB ; Cow.

PubMed:30011095

[IF=0] Garovic, Vesna D., and Muthuvel Jayachandran. "DETECTING PODOCYTE INJURY IN DIABETIC NEPHROPATHY AND GLOMERULONEPHRITIS." U.S. Patent No. 20,170,003,299. 5 Jan. 2017. Human.

PubMed:U.S.PatentNo.20,170,003,299.5