Liver (Mouse) Lysate at 40 ug
Primary: Anti-phospho-PARK2 (Ser378) (SL5553R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD
Sample:
Liver (Mouse) Lysate at 40 ug
Primary: Anti- phospho-PARK2 (Ser378) (SL5553R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD
Sample:
Kidney (Mouse) Lysate at 40 ug
Spleen (Mouse) Lysate at 40 ug
Primary: Anti- phospho-PARK2 (Ser378) (SL5553R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD
Sample:
Lane 1: Mouse Stomach tissue lysates
Lane 2: Human SH-SY5Y cell lysates
Primary: Anti- phospho-PARK2 (Ser378) (SL5553R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kDa
Observed band size: 47 kDa
Sample:
Cerebellum (Mouse) Lysate at 40 ug
Primary: Anti- phospho-PARK2 (Ser378) (SL5553R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-PARK2 (Ser378)) Polyclonal Antibody, Unconjugated (SL5553R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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