Sample:
Hela(Human) Cell Lysate at 30 ug
Primary: Anti-UCP-2 (SL20751R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kD
Observed band size: 34 kD
Sample:
SP2/0 cell(human) Lysate at 30 ug
A673 cell(human) Lysate at 30 ug
Primary: Anti- UCP2 (SL20751R) at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34kD
Observed band size: 34kD
Sample:
Skeletal muscle (Mouse) Lysate at 40 ug
Primary: Anti- UCP-2 (SL20751R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kD
Observed band size: 35 kD
Sample:
TT(Human) Cell Lysate at 30 ug
Primary: Anti-UCP-2 (SL20751R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kD
Observed band size: 34 kD
Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (UCP-2) Polyclonal Antibody, Unconjugated (SL20751R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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