Rabbit Anti-DcR3 antibody | Sunlong Medical

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DcR3; Decoy Receptor 3; Decoy receptor for Fas ligand; DJ583P15.1.1; M68; OTTHUMP00000031583; TNF6B_HUMAN; TNFRSF6B; TR6; Tumor necrosis factor receptor superfamily member 6B; M68; Tumor necrosis factor receptor superfamily member 6b decoy; UNQ186/PRO212.

Cat:

SL23737R

Species Reactivity：

Human,Mouse,(predicted: Dog,Cow,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human DcR3:51-150/300

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Stomach (Mouse) Lysate at 40 ugPrimary: Anti- DcR3 (SL23737R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 30 kDObserved band size: 30 kDSample: Lymph node (Mouse) Lysate at 40 ugSpleen (Mouse) Lysate at 40 ugPrimary: Anti- DcR3 (SL23737R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 30 kDObserved band size: 30 kDSample: DU145(Human) Cell Lysate at 30 ugPrimary: Anti-DcR3 (SL23737R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 30 kDObserved band size: 37 kDSample: Hela(Human) Cell Lysate at 30 ugPrimary: Anti-DcR3 (SL23737R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 30 kDObserved band size: 32 kDParaformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DcR3) Polyclonal Antibody, Unconjugated (SL23737R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors. Several novel members in the TNFR family including DR3, DR4, DR5, and DR6 were recently discovered and function as cell death receptors. Two decoy receptors, DcR1 and DcR2, were recently identified to compete with DR4 and DR5 for their ligand TRAIL binding. A novel decoy receptor was more recently discovered and designated DcR3 and TR6, respectively. Unlike DcR1and DcR2, DcR3 is a soluble rather than a membraneassociated molecule. DcR3 binds to FasL and LIGHT andinhibits FasL and LIGHT induced apoptosis. DcR3 transcript is expressed in a number of lung and coloncarcinomas and in some normal tissues.

Function:
Decoy receptor that can neutralize the cytotoxic ligands TNFS14/LIGHT, TNFSF15 and TNFSF6/FASL. Protects against apoptosis.

Subcellular Location:
Secreted.

Tissue Specificity:
Detected in fetal lung, brain and liver. Detected in adult stomach, spinal cord, lymph node, trachea, spleen, colon and lung. Highly expressed in several primary tumors from colon, stomach, rectum, esophagus and in SW96 colon carcinoma cells.

Similarity:
Contains 4 TNFR-Cys repeats.

SWISS:
O95407

Gene ID:
8771

Database links:

Entrez Gene: 8771 Human

Omim: 603361 Human

SwissProt: O95407 Human

Unigene: 434878 Human

Picture

Sample:
Lymph node (Mouse) Lysate at 40 ug
Spleen (Mouse) Lysate at 40 ug
Primary: Anti- DcR3 (SL23737R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 30 kD
Observed band size: 30 kD

Sample:
DU145(Human) Cell Lysate at 30 ug
Primary: Anti-DcR3 (SL23737R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 30 kD
Observed band size: 37 kD

Sample:
Hela(Human) Cell Lysate at 30 ug
Primary: Anti-DcR3 (SL23737R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 30 kD
Observed band size: 32 kD

Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DcR3) Polyclonal Antibody, Unconjugated (SL23737R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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