Rabbit Anti-Adiponectin Receptor 1 antibody

Home > Product > Antibody > Rabbit Anti-Adiponectin Receptor 1 antibody

ACDCR1; ADIPO R1; Adiponectin receptor protein 1; ADIPOR 1; ADIPOR1; CGI-45; CGI-45 protein;FLJ25385; PAQR1; Progestin and adipoQ receptor family member I; TESBP1A; ADR1_HUMAN.

Cat:

SL0610R

Species Reactivity：

Human,Mouse,Rat,(predicted: Chicken,Dog,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human Adiponectin Receptor 1:241-270/375<Cytoplasmic>

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Muscle(Mouse) Lysate at 40 ugPrimary: Anti- Adiponectin Receptor 1 (SL0610R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 42 kDObserved band size: 42 kDSample: Lane 1: Mouse Muscle tissue lysates Lane 2: Mouse Breast tissue lysates Lane 3: Mouse Heart tissue lysates Lane 4: Rat Muscle tissue lysates Lane 5: Rat Heart tissue lysates Primary: Anti-Adiponectin Receptor 1 (SL0610R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 42 kDaObserved band size: 42 kDaTissue/cell: rat pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37∩ for 20 min; Incubation: Anti-Adiponectin Receptor 1 Polyclonal Antibody, Unconjugated(SL0610R) 1:300, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingTissue/cell: human rectal tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37∩ for 20 min; Incubation: Anti-Adiponectin Receptor 1 Polyclonal Antibody, Unconjugated(SL0610R) 1:300, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Acrp30 is a hormone secreted by adipocytes that acts as an antidiabetic and anti-atherogenic adipokine. Levels of adiponectin in the blood are decreased under conditions of obesity, insulin resistance and type 2 diabetes. Administration of adiponectin causes glucose-lowering effects and ameliorates insulin resistance in mice. Conversely, adiponectin-deficient mice exhibit insulin resistance and diabetes. This insulin-sensitizing effect of adiponectin seems to be mediated by an increase in fatty-acid oxidation through activation of AMP kinase and PPAR- . Cloning of complementary DNAs encoding adiponectin receptors 1 and 2 (AdipoR1 and AdipoR2) have shown that AdipoR1 is abundantly expressed in skeletal muscle, whereas AdipoR2 is predominantly expressed in the liver.

Function:
Receptor for globular and full-length adiponectin (APM1), an essential hormone secreted by adipocytes that acts as an antidiabetic. Probably involved in metabolic pathways that regulate lipid metabolism such as fatty acid oxidation. Mediates increased AMPK, PPARA ligand activity, fatty acid oxidation and glucose uptake by adiponectin. Has some high-affinity receptor for globular adiponectin but low-affinity receptor for full-length adiponectin.

Subunit:
May form homo and heteromultimers.

Subcellular Location:
Membrane; Multi-pass membrane protein. Note=Localized to the cell membrane and intracellular organelles.

Tissue Specificity:
Widely expressed. Highly expressed in skeletal muscle. Expressed at intermediate level in brain, heart, spleen, kidney, liver, placenta, lung and peripheral blood leukocytes. Weakly expressed in colon, thymus and small intestine.

Similarity:
Belongs to the ADIPOR family.

SWISS:
Q96A54

Gene ID:
51094

Database links:

Entrez Gene: 51094 Human

Entrez Gene: 72674 Mouse

Entrez Gene: 289036 Rat

Omim: 607945 Human

SwissProt: Q96A54 Human

SwissProt: Q91VH1 Mouse

Unigene: 5298 Human

Unigene: 259976 Mouse

脂联素与受体结合后具有增强胰岛素敏感性，抗高血糖，抗动脉粥样硬化等生物学效应，任何增加或减少脂联素及其受体表达的方法都影响这些疾病的发生和发展.
脂联素受体-1(AdipoR1和AdipoR2)同属于PAQR家族，具有七次跨膜蛋白的特有结构，
mol wt:41kDa Picture

Sample:
Lane 1: Mouse Muscle tissue lysates
Lane 2: Mouse Breast tissue lysates
Lane 3: Mouse Heart tissue lysates
Lane 4: Rat Muscle tissue lysates
Lane 5: Rat Heart tissue lysates
Primary: Anti-Adiponectin Receptor 1 (SL0610R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa

Tissue/cell: rat pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37∩ for 20 min;
Incubation: Anti-Adiponectin Receptor 1 Polyclonal Antibody, Unconjugated(SL0610R) 1:300, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Tissue/cell: human rectal tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37∩ for 20 min;
Incubation: Anti-Adiponectin Receptor 1 Polyclonal Antibody, Unconjugated(SL0610R) 1:300, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Product Feedback Wall

Specific References (13) | SL0610R has been referenced in 13 publications.

[IF=5.037] Annamária Schaffer. et al. The ontogenies of endometrial and myometrial leptin and adiponectin receptors in pregnant rats: Their putative impact on uterine contractility. Life Sci. 2022 May;297:120465 WB ; Rat.

PubMed:35271883

[IF=1.28] Li, Jiehua, Shiyue Su, and Xiaona Zong. "Analysis of the association between adiponectin, adiponectin receptor 1 and diabetic cardiomyopathy."Experimental and therapeutic medicine 7.4 (2014): 1023-1027. IHSLCP ; Rat.

PubMed:24669271

[IF=5.14] Wu, Ming-Hsiu, et al. "Obesity Exacerbates Rat Cerebral Ischemic Injury through Enhancing Ischemic Adiponectin-Containing Neuronal Apoptosis."Molecular Neurobiology (2015): 1-12. WB ; Rat.

PubMed:26126515

[IF=11.09] Wang, Yajing, et al. "GRK2-Mediated Desensitization of AdipoR1 in Failing Heart." Circulation (2015): CIRCULATIONAHA-114. IP ; Mouse.

PubMed:25696921

[IF=2.51] Ji, Hongfei, et al. "The effect of resveratrol on the expression of AdipoR1 in kidneys of diabetic nephropathy." Molecular Biology Reports: 1-9. IHSLCP ; Rat.

PubMed:24413998

[IF=1.92] Guo, Zhixin, et al. "Effect of exenatide on the cardiac expression of adiponectin receptor 1 and NADPH oxidase subunits and heart function in streptozotocin-induced diabetic rats." Diabetology & Metabolic Syndrome 6.1 (2014): 29. IHSLCP ; Rat.

PubMed:24576329

[IF=2.952] Ruiying Liu. et al. Adiponectin inhibits D‑gal‑induced cardiomyocyte senescence via AdipoR1/APPL1. Mol Med Rep. 2021 Oct;24(4):1-10 WB ; Mouse.

PubMed:34396435

[IF=6.304] Jia Gao. et al. Nicotine aggravates vascular adiponectin resistance via ubiquitin-mediated adiponectin receptor degradation in diabetic Apolipoprotein E knockout mouse. Cell Death Dis. 2021 May;12(6):1-12 WB,IF ; Human.

PubMed:34006831

[IF=4.75] Guo et al. Effect of telmisartan on the expression of adiponectin receptors and nicotinamide adenine dinucleotide phosphate oxidase in the heart and aorta in type 2 diabetic rats. (2012) Cardiovasc.Diabetol. 11:94 IHC ; Rat.

PubMed:22873349

[IF=2.23] Cao, Zhongzan, et al. "Molecular cloning and expression analysis of adiponectin and its receptors (AdipoR1 and AdipoR2) in the hypothalamus of the Huoyan goose during different stages of the egg-laying cycle."Reproductive Biology and Endocrinology 13.1 (2015): 87. WB ;

PubMed:26251033

[IF=5.74] Liu, Gai-Zhen, et al. "High glucose/High Lipids impair vascular adiponectin function via inhibition of caveolin-1/AdipoR1 signalsome formation." Free Radical Biology and Medicine (2015). WB ; Human.

PubMed:26453924

[IF=6.43] Morad, Vivian, Annelie Abrahamsson, and Charlotta Dabrosin. "Estradiol affects extracellular leptin: adiponectin ratio in human breast tissue in vivo." The Journal of Clinical Endocrinology & Metabolism (2014). IHSLCP ; Human.

PubMed:24796929

[IF=2.59] Liu, Su, et al. "The ameliorating effect of rosiglitazone on experimental nonalcoholic steatohepatitis is associated with regulating adiponectin receptor expression in rats." European journal of pharmacology 650.1 (2011): 384. WB ; Rat.

PubMed:20965162