Rabbit Anti-c-fos antibody | Sunlong Medical

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Cellular oncogene fos; FBJ murine osteosarcoma viral v fos oncogene homolog antibody FBJ Osteosarcoma Virus; FOS; FOS protein; G0 G1 switch regulatory protein 7; G0S7; Oncogene FOS; Proto oncogene protein c fos; v fos FBJ murine osteosarcoma viral oncogen

Cat:

SL0469R

Species Reactivity：

Human,Mouse,Rat,(predicted: Pig,)

Immunogen：

KLH conjugated synthetic peptide derived from human c-fos:1-100/380

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

MCF-7 Cell (Human) Lysate at 30 ugEmbryo(Mouse) Lysate at 40 ugRAW246.7(Mouse)Lysate at 30 ugNIH/3T3(Mouse)Lysate at 30 ugCerebrum(Mouse) Lysate at 40 ugCerebrum(Rat) Lysate at 40 ugPrimary: Anti- c-fos (SL0469R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 41kDObserved band size: 55KDParaformaldehyde-fixed, paraffin embedded (Human esophageal cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (c-fos) Polyclonal Antibody, Unconjugated (SL0469R) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (human laryngeal carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (c-fos) Polyclonal Antibody, Unconjugated (SL0469R) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (c-fos) Polyclonal Antibody, Unconjugated (SL0469R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat spinal cord); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (c-fos) Polyclonal Antibody, Unconjugated (SL0469R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (c-fos) Polyclonal Antibody, Unconjugated (SL0469R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (c-fos) Polyclonal Antibody, Unconjugated (SL0469R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Tissue/cell:MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, SLC0005) at 37°C for 20 min; Antibody incubation with (c-fos) polyclonal Antibody, Unconjugated (SL0469R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.Tissue/cell: A431 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, SLC0005) at 37°C for 20 min; Antibody incubation with (c-fos) polyclonal Antibody, Unconjugated (SL0469R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death. [provided by RefSeq, Jul 2008].

Function:
Nuclear phosphoprotein which forms a tight butnon-covalently linked complex with the JUN/AP-1 transcriptionfactor. In the heterodimer, FOS and JUN/AP-1 basic regions eachseems to interact with symmetrical DNA half sites. On TGF-betaactivation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at theAP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Hasa critical function in regulating the Has a critical function inregulating the development of cells destined to form and maintainthe skeleton. It is thought to have an important role in signaltransduction, cell proliferation and differentiation.

Subunit:
Heterodimer; with JUN (By similarity). Interacts withMAFB. Component of the SMAD3/SMAD4/JUN/FOS complexrequired for syngernistic TGF-beta-mediated transcription at theAP1 promoter site. Interacts with SMAD3; the interaction is weakeven on TGF-beta activation. Interacts with MAFB. Interacts withDSIPI; this interaction inhibits the binding of active AP1 to itstarget DNA.

Subcellular Location:
Nucleus.

Post-translational modifications:
Phosphorylated in the SLCterminal upon stimulation by nervegrowth factor (NGF) and epidermal growth factor (EGF).Phosphorylated, in vitro, by MAPK and RSK1. Phosphorylation on bothSer-362 and Ser-374 by MAPK1/2 and RSK1/2 leads to proteinstabilization with phosphorylation on Ser-374 being the major sitefor protein stabilization on NGF stimulation. Phosphorylation onSer-362 and Ser-374 primes further phosphorylations on Thr-325 andThr-331 through promoting docking of MAPK to the DEF domain.Phosphorylation on Thr-232, induced by HA-RAS, activates thetranscriptional activity and antagonizes sumoylation.Phosphorylation on Ser-362 by RSK2 in osteoblasts contributes toosteoblast transformation (By similarity). [PTM] Constitutively sumoylated by SUMO1, SUMO2 and SUMO3.Desumoylated by SENP2. Sumoylation requires heterodimerization withJUN and is enhanced by mitogen stimulation. Sumoylation inhibitsthe AP-1 transcriptional activity and is, itself, inhibited byRas-activated phosphorylation on Thr-232.

Similarity:
Belongs to the bZIP family. Fos subfamily.
Contains 1 bZIP domain.

SWISS:
P01100

Gene ID:
2353

Database links:

Entrez Gene: 2353 Human

Entrez Gene: 14281 Mouse

Entrez Gene: 314322 Rat

Omim: 164810 Human

SwissProt: P01100 Human

SwissProt: P01101 Mouse

SwissProt: P12841 Rat

Unigene: 246513 Mouse

Unigene: 103750 Rat

c-fos的作用主要用于各种类型的恶性肿瘤如食管癌、鼻咽癌、乳腺癌、结肠癌以及脑病的研究。
c-fos原癌基因及其蛋白产物不仅参与细胞的正常生长、分化过程，而且也参与细胞内信息传递过程和细胞的能量代谢过程，对细胞的增生、分化、转化都有调节作用、在生命活动中起着极为基础而重要的作用。 Picture

Paraformaldehyde-fixed, paraffin embedded (Human esophageal cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (c-fos) Polyclonal Antibody, Unconjugated (SL0469R) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human laryngeal carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (c-fos) Polyclonal Antibody, Unconjugated (SL0469R) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (c-fos) Polyclonal Antibody, Unconjugated (SL0469R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat spinal cord); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (c-fos) Polyclonal Antibody, Unconjugated (SL0469R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (c-fos) Polyclonal Antibody, Unconjugated (SL0469R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (c-fos) Polyclonal Antibody, Unconjugated (SL0469R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Tissue/cell:MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, SLC0005) at 37°C for 20 min; Antibody incubation with (c-fos) polyclonal Antibody, Unconjugated (SL0469R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Tissue/cell: A431 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, SLC0005) at 37°C for 20 min; Antibody incubation with (c-fos) polyclonal Antibody, Unconjugated (SL0469R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

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Specific References (9) | SL0469R has been referenced in 9 publications.

[IF=2.562] IHC ; rat.

PubMed:32731263

[IF=5.639] Jing Wen. et al. The cAMP Response Element- Binding Protein/Brain-Derived Neurotrophic Factor Pathway in Anterior Cingulate Cortex Regulates Neuropathic Pain and Anxiodepression Like Behaviors in Rats. Front Mol Neurosci. 2022; 15: 831151 WB ; Rat.

PubMed:35401106

[IF=2.014] Li LI. et al. The antibacterial activity of Berberis heteropoda Schrenk and its effect on irritable bowel syndrome in rats. Chin J Nat Medicines. 2020 May;18:356 IHC ; Rat.

PubMed:10.1016/S1875-5364(20)30042-X

[IF=3.169] Qinjun Chu. et al. A Single Low Dose of Dexmedetomidine Efficiently Attenuates Esketamine-Induced Overactive Behaviors and Neuronal Hyperactivities in Mice. Front Hum Neurosci. 2021; 15: 735569 WB,IHC ; Mice .

PubMed:34712126

[IF=2.733] Bei Miao. et al. Protective effect of HCN2-induced SON sensitization on chronic visceral hypersensitivity in neonatal-CRD rat model. Brain Res. 2021 Sep;1767:147538 IF ; Rat.

PubMed:34052259

[IF=3.266] Ma Q et al. Vitamin B5 inhibit RANKL induced osteoclastogenesis and ovariectomy induced osteoporosis by scavenging ROS generation. Am J Transl Res. 2019 Aug 15;11(8):5008-5018. eCollection 2019. WB ; Mouse.

PubMed:31497217

[IF=6.551] Mei Ha. et al. PKCα mediated by the PI3K/Akt-FOXA1 cascade facilitates cypermethrin-induced hyperthyroidism. Sci Total Environ. 2021 Feb;757:143727 WB ; Rat.

PubMed:33250241

[IF=12.279] Zhang Q et al. Photoactivatable Prodrug-Backboned Polymeric Nanoparticles for Efficient Light-Controlled Gene Delivery and Synergistic Treatment of Platinum-Resistant Ovarian Cancer. Nano Lett. 2020 Apr 9. WB&ICF ; Human.

PubMed:32250633

[IF=4.4] Xiao, Jinyu, et al. "Effects of GABA microinjection into dorsal raphe nucleus on behavior and activity of lateral habenular neurons in mice." Experimental Neurology (2017).

PubMed:28843542