Rabbit Anti-CXorf36 antibody

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Deleted in autism 1 related protein; DIA1R; EPQL1862; hCG_1981635; hCG1981635; PRO3743; UPF0672 protein CXorf36; chromosome X open reading frame 36, isoform CRA_a; uncharacterized protein Cxorf36 precursor; PRO3743; EPQL1862; FLJ14103; FLJ55198; FLJ55198,

Cat:

SL0727R

Species Reactivity：

Mouse,(predicted: Human,)

Immunogen：

KLH conjugated synthetic peptide derived from human CXorf36:101-182/182

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Cerebrum (Mouse) Lysate at 40 ugPrimary: Anti-CXorf36 (SL0727R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 45 kDObserved band size: 50 kDSample: Kidney(Mouse) lysate at 60ug; Liver(Mouse) lysate at 60ug;Primary: Anti-CXorf36 (SL0727R) at 1:300; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(SL0295G-HRP) at 1: 5000; Predicted band size :45 kDObserved band size :50/75 kDSample: Lung (Mouse) Lysate at 40 ugPrimary: Anti-CXorf36 (SL0727R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 45 kDObserved band size: 50 kD Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CXorf36) Polyclonal Antibody, Unconjugated (SL0727R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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The X and Y chromosomes are the human sex chromosomes. Chromosome X consists of about 153 million base pairs and nearly 1,000 genes. The combination of an X and Y chromosome lead to normal male development while two copies of X lead to normal female development. There are a number of conditions related to an unsual number and combination of sex chromosomes being inherited. More than one copy of the X chromosome with a Y chromosome causes Klinefelter's syndrome. A single copy of X alone leads to Turner's syndrome. More than 2 copies of the X chromosome, in the absence of a Y chromosome, is known as Triple X syndrome. Color blindness, hemophilia, and Duchenne muscular dystrophy are well known X chromosome-linked conditions which affect males more frequently as males carry a single X chromosome. The CXorf36 gene product has been provisionally designated CXorf36 pending further characterization.

Subcellular Location:
Secreted (Potential).

DISEASE:
Note=Genetic variations in CXorf36 may be associated with susceptibility to autism.

Similarity:
Belongs to the DIA1 family.

SWISS:
Q9H7Y0

Gene ID:
79742

Database links:

Entrez Gene: 79742 Human

SwissProt: Q9H7Y0 Human

Unigene: 98321 Human

Picture

Sample:
Kidney(Mouse) lysate at 60ug;
Liver(Mouse) lysate at 60ug;
Primary: Anti-CXorf36 (SL0727R) at 1:300;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(SL0295G-HRP) at 1: 5000;
Predicted band size :45 kD
Observed band size :50/75 kD

Sample:
Lung (Mouse) Lysate at 40 ug
Primary: Anti-CXorf36 (SL0727R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kD
Observed band size: 50 kD

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CXorf36) Polyclonal Antibody, Unconjugated (SL0727R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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