Rabbit Anti-SYVN1 antibody | Sunlong Medical

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1200010C09Rik; HRD1; KIAA1810; MGC40372; Synovial apoptosis inhibitor 1 synoviolin; Synoviolin 1 isoform b; SYVN1_HUMAN; DER3; E3 ubiquitin-protein ligase synoviolin; HMG coA reductase degradation 1 homolog; OTTHUMP00000230429; OTTHUMP00000230430; OTTHUMP

Cat:

SL0679R

Species Reactivity：

Human,Mouse,(predicted: Rat,Dog,Cow,)

Immunogen：

KLH conjugated synthetic peptide derived from human SYVN1:531-617/617

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=3μg/TestIF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Kidney (Mouse) Lysate at 40 ugLiver (Mouse) Lysate at 40 ugPrimary: Anti-SYVN1 (SL0679R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 65 kDObserved band size: 65/70 kDTissue/cell: mouse kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-SYVN1/HRD1 Polyclonal Antibody, Unconjugated(SL0679R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingTissue/cell: mouse liver tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-SYVN1/HRD1 Polyclonal Antibody, Unconjugated(SL0679R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingThe figure annotation: The blue histogram is unstained cells.The Wathet Blue histogram is cells stained with secondary antibody (SL0295G-FITC) alone.The Orange histogram is cells stained with rabbit IgG isotype control antibody(SL0295P)plus secondary antibody. The green histogram is cells stained with Rabbit Anti-SYVN1 antibody (SL0679R) plus secondary antibody.Positive control: Hepg2 cells Concentration: 1:50

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This gene encodes a protein involved in endoplasmic reticulum (ER)-associated degradation. The encoded protein removes unfolded proteins, accumulated during ER stress, by retrograde transport to the cytosol from the ER. This protein also uses the ubiquitin-proteasome system for additional degradation of unfolded proteins. Sequence analysis identified two transcript variants that encode different isoforms. [provided by RefSeq, May 2011]

Function:
Acts as an E3 ubiquitin-protein ligase which accepts ubiquitin specifically from endoplasmic reticulum-associated UBC7 E2 ligase and transfers it to substrates, promoting their degradation. Component of the endoplasmic reticulum quality control (ERQC) system also called ER-associated degradation (ERAD) involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins. Also promotes the degradation of normal but naturally short-lived proteins such as SGK. Protects cells from ER stress-induced apoptosis. Protects neurons from apoptosis induced by polyglutamine-expanded huntingtin (HTT) or unfolded GPR37 by promoting their degradation. Sequesters p53/TP53 in the cytoplasm and promotes its degradation, thereby negatively regulating its biological function in transcription, cell cycle regulation and apoptosis.

Subunit:
Homodimer. Interacts with p53/TP53 and HTT. Interacts with VCP, HERPUD1 and DERL1. Part of a complex containing SYVN1, HERPUD1, SELS and DERL1; which probably transfer misfolded proteins from the ER to VCP. Part of a complex containing SYVN1, SEL1L and DERL2. Interacts with UBXN6. Interacts with SEL1L; recruits ERLEC1 and OS9. May form a complex with ERLEC1; HSPA5; OS9 AND SEL1L.

Subcellular Location:
Endoplasmic reticulum membrane; Multi-pass membrane protein.

Tissue Specificity:
Ubiquitously expressed, with highest levels in liver and kidney (at protein level). Up-regulated in synovial tissues from patients with rheumatoid arthritis (at protein level).

Post-translational modifications:
Not N-glycosylated.
Auto-ubiquitinated.

Similarity:
Belongs to the HRD1 family.
Contains 1 RING-type zinc finger.

SWISS:
Q86TM6

Gene ID:
84447

Database links:

Entrez Gene: 84447 Human

Entrez Gene: 74126 Mouse

Entrez Gene: 361712 Rat

Omim: 608046 Human

SwissProt: Q86TM6 Human

SwissProt: Q9DBY1 Mouse

Unigene: 75859 Human

Unigene: 149870 Mouse

与关节滑膜的损伤有关。 Picture

Tissue/cell: mouse kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-SYVN1/HRD1 Polyclonal Antibody, Unconjugated(SL0679R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Tissue/cell: mouse liver tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-SYVN1/HRD1 Polyclonal Antibody, Unconjugated(SL0679R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

The figure annotation:
The blue histogram is unstained cells.
The Wathet Blue histogram is cells stained with secondary antibody (SL0295G-FITC) alone.
The Orange histogram is cells stained with rabbit IgG isotype control antibody(SL0295P)plus secondary antibody.
The green histogram is cells stained with Rabbit Anti-SYVN1 antibody (SL0679R) plus secondary antibody.
Positive control: Hepg2 cells
Concentration: 1:50

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