Testis and spermatogenesis related gene 4 may play a role in the development of cryptorchidism, the failure of the testis to descend from the abdomen into the scrotum [RGD] [TISSUE SPECIFICITY] Testis.
Tissue Specificity:
Widely expressed.
Similarity:
Contains 1 SUN domain.
SWISS:
Q8TC36
Gene ID:
140732
Database links:
Entrez Gene: 140732 Human
NCBI: NP_54486 Human
SwissProt: Q8TC36 Human
生精细胞凋亡相关基因TSARG4与精原细胞的生成和凋亡有关。
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Sample:Brain(Mouse) Lysate at 30 ug
Primary: Anti-SPAG4L (SL1220R) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G-HRP) at 1: 5000 dilution;
Predicted band size : 42 kD
Observed band size : 42 kD
Tissue/cell: Rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-SPAG4L Polyclonal Antibody, Unconjugated(SL1220R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
Tissue/cell: mouse stomach tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-SRG4/TSARG4 Polyclonal Antibody, Unconjugated(SL1220R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
Tissue/cell: rat pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-SRG4/TSARG4 Polyclonal Antibody, Unconjugated(SL1220R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
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