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Home > Product > Antibody > Rabbit Anti-WNT5A antibody
wingless-related MMTV integration site 5A; hWNT 5A; hWNT5A; Protein Wnt 5a; Protein Wnt5a; Wingless type MMTV integration site family member 5A; Wnt-5a; WNT 5A protein precursor; WNT5A protein precursor; WNT5A_HUMAN.
Cat:
SL1948R
Species Reactivity：
Human,Mouse,Rat,(predicted: Pig,Cow,Rabbit,)
Immunogen：
KLH conjugated synthetic peptide derived from human WNT5A:301-381/381
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=1ug/TestIF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Sample:Uters(Mouse) Lysate at 30 ugPrimary: Anti-WNT5A (SL1948R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilutionPredicted band size: 35 kDObserved band size: 47 kDSample:Ovary(Mouse) Lysate at 30 ugPrimary: Anti-WNT5A (SL1948R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilutionPredicted band size: 35 kDObserved band size: 47 kDSample: Lane 1: Embryo (Mouse) Lysate at 40 ugLane 2: Cerebrum (Mouse) Lysate at 40 ugLane 3: Uterus (Mouse) Lysate at 40 ugLane 4: Uterus (Rat) Lysate at 40 ugLane 5: Ovary (Rat) Lysate at 40 ugLane 6: Vas deferens (Rat) Lysate at 40 ugPrimary: Anti-WNT5A (SL1948R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 42 kDObserved band size: 42 kDParaformaldehyde-fixed, paraffin embedded (rat ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (WNT5A) Polyclonal Antibody, Unconjugated (SL1948R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Images provided the Independent Validation Program (badge number 029641)Formalin-fixed and paraffin embedded human breast labeled with Rabbit Anti-WNT5A Polyclonal Antibody (SL1948R) at 1:250 at room temperature overnight followed by conjugation to secondary antibody.Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37∩ for 20 min; Incubation: Anti-WNT5A Polyclonal Antibody, Unconjugated(SL1948R) 1:200, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingBlank control: HepG2. Primary Antibody (green line): Rabbit Anti-WNT5A antibody (SL1948R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647Dilution: 1μg /test. ProtocolThe cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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Datasheet:


The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene encodes a member of the WNT family that signals through both the canonical and non-canonical WNT pathways. This protein is a ligand for the seven transmembrane receptor frizzled-5 and the tyrosine kinase orphan receptor 2. This protein plays an essential role in regulating developmental pathways during embryogenesis. This protein may also play a role in oncogenesis. Mutations in this gene are the cause of autosomal dominant Robinow syndrome. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012].

Function:
Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene expression. Suppression of the canonical pathway allows chondrogenesis to occur and inhibits tumor formation. Stimulates cell migration. Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor. Mediates motility of melanoma cells. Required during embryogenesis for extension of the primary anterior-posterior axis and for outgrowth of limbs and the genital tubercle. Inhibits type II collagen expression in chondrocytes.

Subunit:
Interacts with PORCN. Interacts with WLS.

Subcellular Location:
Secreted, extracellular space, extracellular matrix.

Tissue Specificity:
Expression is increased in differentiated thyroid carcinomas compared to normal thyroid tissue and anaplastic thyroid tumors where expression is low or undetectable. Expression is found in thyrocytes but not in stromal cells (at protein level).

Post-translational modifications:
Palmitoylation is necessary for stimulation of cell migration, inhibition of the beta-catenin pathway and receptor binding.
Glycosylation is necessary for secretion but not for activity.

DISEASE:
Defects in WNT5A are the cause of Robinow syndrome autosomal dominant (DRS) [MIM:36700]. A disease characterized by short-limb dwarfism, costovertebral segmentation defects and abnormalities of the head, face and external genitalia. The clinical signs are generally milder in dominant cases.

Similarity:
Belongs to the Wnt family.

SWISS:
P41221

Gene ID:
7474

Database links:

Entrez Gene: 7474 Human

Entrez Gene: 530005 Cow

Entrez Gene: 22418 Mouse

Entrez Gene: 64566 Rat

Omim: 164975 Human

SwissProt: P41221 Human

SwissProt: P22725 Mouse

SwissProt: Q5PY99 Rat

SwissProt: Q9QXQ7 Rat

Unigene: 643085 Human

Unigene: 287544 Mouse

Unigene: 48749 Rat



WNT5A蛋白属于Wnt原癌基因家族中的一种。Wnt5a与肿瘤、发生、转移有关。 Picture

Sample:Uters(Mouse) Lysate at 30 ug
Primary: Anti-WNT5A (SL1948R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 35 kD
Observed band size: 47 kD
Sample:Ovary(Mouse) Lysate at 30 ug
Primary: Anti-WNT5A (SL1948R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 35 kD
Observed band size: 47 kD
Sample:
Lane 1: Embryo (Mouse) Lysate at 40 ug
Lane 2: Cerebrum (Mouse) Lysate at 40 ug
Lane 3: Uterus (Mouse) Lysate at 40 ug
Lane 4: Uterus (Rat) Lysate at 40 ug
Lane 5: Ovary (Rat) Lysate at 40 ug
Lane 6: Vas deferens (Rat) Lysate at 40 ug
Primary: Anti-WNT5A (SL1948R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 42 kD
Paraformaldehyde-fixed, paraffin embedded (rat ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (WNT5A) Polyclonal Antibody, Unconjugated (SL1948R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Images provided the Independent Validation Program (badge number 029641)Formalin-fixed and paraffin embedded human breast labeled with Rabbit Anti-WNT5A Polyclonal Antibody (SL1948R) at 1:250 at room temperature overnight followed by conjugation to secondary antibody.
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37∩ for 20 min;
Incubation: Anti-WNT5A Polyclonal Antibody, Unconjugated(SL1948R) 1:200, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
Blank control: HepG2.
Primary Antibody (green line): Rabbit Anti-WNT5A antibody (SL1948R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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