Rabbit Anti-CXCL16 antibody

Home > Product > Antibody > Rabbit Anti-CXCL16 antibody

CXC-chemokine ligand 16; Chemokine (C X C motif) ligand 16; CXC chemokine ligand 16; CXCLG16; Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein; SCYB16; Small inducible cytokine B16 precursor; SRPSOX; Transmembrane chemokine C

Cat:

SL1441R

Species Reactivity：

Human,Mouse,(predicted: Rat,)

Immunogen：

KLH conjugated synthetic peptide derived from the middle of human CXCL16:85-200/247

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=3ug/TestIF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-CXCL16 Polyclonal Antibody, Unconjugated(SL1441R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingImages kindly provided by Dr. Denny Cottle from the publication, Fetal inhibition of inflammation improves disease phenotypes in Harlequin Ichthyosis.Formalin-fixed and paraffin embedded mouse skin probed with Rabbit Anti-CXCL16 Polyclonal Antibody (SL1441R) at 1:50 for two hours at room temperature (green channel).Blank control: A431. Primary Antibody (green line): Rabbit Anti-CXCL16 antibody (SL1441R) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody: Goat anti-rabbit IgG-AF647Dilution: 3μg /test. ProtocolThe cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

prev next

Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
Feedback Wall

CXCL16 acts as a scavenger receptor on macrophages, which specifically binds to OxLDL (oxidized low density lipoprotein), suggesting that it may be involved in pathophysiology such as atherogenesis. It induces a strong chemotactic response and calcium mobilization. It binds to CXCR6/Bonzo.

Subcellular Location:
Cell membrane. Secreted. Also exists as a soluble form.

Tissue Specificity:
Expressed in T-cell areas. Expressed in spleen, lymph nodes, lung, kidney, small intestine and thymus. Weak expression in heart and liver and no expression in brain and bone marrow.

Similarity:
Belongs to the intercrine alpha (chemokine CxC) family.

SWISS:
Q8BSU2

Gene ID:
58191

Database links:

Entrez Gene: 58191 Human

Omim: 605398 Human

SwissProt: Q9H2A7 Human

Unigene: 513885 Human

Picture

Images kindly provided by Dr. Denny Cottle from the publication, Fetal inhibition of inflammation improves disease phenotypes in Harlequin Ichthyosis.Formalin-fixed and paraffin embedded mouse skin probed with Rabbit Anti-CXCL16 Polyclonal Antibody (SL1441R) at 1:50 for two hours at room temperature (green channel).

Blank control: A431.
Primary Antibody (green line): Rabbit Anti-CXCL16 antibody (SL1441R)
Dilution: 3μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 3μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Product Feedback Wall