Rabbit Anti-Wnt3a antibody | Sunlong Medical

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MGC119418; MGC119419; MGC119420; Protein Wnt 3a Precursor; Protein Wnt-3a; Wingless type MMTV integration site family member 3A; Wnt 3a; WNT3A protein.

Cat:

SL1700R

Species Reactivity：

Human,Mouse,Rat,(predicted: Pig,)

Immunogen：

KLH conjugated synthetic peptide derived from human Wnt3a:281-352/352

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample:U2os Cell(Human)Lysate at 30 ugPrimary: Anti- Wnt3a (SL1700R)at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 37kDObserved band size: 37kDSample: Brain(Rat) lysate at 30ug; Uterus(Mouse ) lysates, 30ug; Primary: Anti-Wnt3a (SL1700R) at 1:200 dilution; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G) at 1: 3000 dilution; Predicted band size : 37kDObserved band size : 37kDImages provided the Independent Validation Program (badge number 029652)Formalin-fixed and paraffin embedded human small intestine labeled with Rabbit Anti-Wnt3a Polyclonal Antibody (SL1700R) at 1:250 overnight at room temperature followed by conjugation to secondary antibody.Tissue/cell: rat ovary tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-Wnt3a Polyclonal Antibody, Unconjugated(SL1700R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 96% amino acid identity to mouse Wnt3A protein, and 84% to human WNT3 protein, another WNT gene product. This gene is clustered with WNT14 gene, another family member, in chromosome 1q42 region. [provided by RefSeq, Jul 2008]

Function:
Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube.

Subunit:
Interacts with PORCN. Interacts with APCDD1 and WLS. Component of the Wnt-Fzd-LRP5-LRP6 signaling complex that contains a WNT protein, a FZD protein and LRP5 or LRP6. Interacts directly in the complex with LRP6.

Subcellular Location:
Secreted, extracellular space, extracellular matr

Tissue Specificity:
Moderately expressed in placenta and at low levels in adult lung, spleen, and prostate.

Post-translational modifications:
Palmitoylation at Ser-209 by PORCN is required for proper trafficking to cell surface, vacuolar acidification is critical to release palmitoylated WNT3A from WLS in secretory vesicles.

Similarity:
Belongs to the Wnt family.

SWISS:
P56704

Gene ID:
89780

Database links:

Entrez Gene: 89780 Human

Entrez Gene: 22416 Mouse

Entrez Gene: 303181 Rat

Omim: 606359 Human

SwissProt: P56704 Human

SwissProt: P27467 Mouse

Unigene: 336930 Human

Unigene: 1367 Mouse

Unigene: 130971 Rat

Wnt3a是Wnt家族的成员之一,对神经干细胞的增殖及分化有一定的调控作用,Wnt3a蛋白可以促进神经干细胞向神经元分化. Picture

Sample:
Brain(Rat) lysate at 30ug;
Uterus(Mouse ) lysates, 30ug;
Primary: Anti-Wnt3a (SL1700R) at 1:200 dilution;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G) at 1: 3000 dilution;
Predicted band size : 37kD
Observed band size : 37kD

Images provided the Independent Validation Program (badge number 029652)Formalin-fixed and paraffin embedded human small intestine labeled with Rabbit Anti-Wnt3a Polyclonal Antibody (SL1700R) at 1:250 overnight at room temperature followed by conjugation to secondary antibody.

Tissue/cell: rat ovary tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-Wnt3a Polyclonal Antibody, Unconjugated(SL1700R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Specific References (15) | SL1700R has been referenced in 15 publications.

[IF=4.486] Xu T et al. MicroRNA‐708 modulates Hepatic Stellate Cells activation and enhances extracellular matrix accumulation via direct targeting TMEM88. J Cell Mol Med . 2020 Jul;24(13):7127-7140. WB ; Human.

PubMed:32463570

[IF=1.92] Yang et al. MicroRNA-195 acts as a tumor suppressor by directly targeting Wnt3a in HepG2 hepatocellular carcinoma cells. (2014) Mol.Med.Rep. 10:2643-8 WB ; Human.

PubMed:25174704

[IF=5.97] Sun et al. miR-491-5p, mediated by Foxi1, functions as a tumor suppressor by targeting Wnt3a/β-catenin signaling in the development of gastric cancer. (2017) Cell.Death.Dis. 8:e2714 IHC,WB ; Human.

PubMed:28358374

[IF=1.26] Guo, Furong, et al. "Snail1 is positively correlated with atrial fibrosis in patients with atrial fibrillation and rheumatic heart disease." Experimental and Therapeutic Medicine.(2017) WB ; Human.

PubMed:29104639

[IF=2.37] Hayashi, Kouhei, et al. "Involvement of the T-box transcription factor Brachyury in early-stage embryonic mouse salivary gland." Biochemical and Biophysical Research Communications (2016). WB ; Mouse.

PubMed:27369076

[IF=2.698] Yanyan Xing. et al. Effects of mechanosensitive ion channel Piezo1 on proliferation and osteogenic differentiation of human dental follicle cells. Ann Anat. 2021 Oct;:151847 WB ; Human.

PubMed:34687906

[IF=13.273] Xinkun Shen. et al. Improvement of aqueous stability and anti-osteoporosis properties of Zn-MOF coatings on titanium implants by hydrophobic raloxifene. Chem Eng J. 2021 Oct;:133094 WB ; Mouse.

PubMed:10.1016/j.cej.2021.133094

[IF=4.225] Yang Fan. et al. Catalpol Protects Against Pulmonary Fibrosis Through Inhibiting TGF-β1/Smad3 and Wnt/β-Catenin Signaling Pathways. Front Pharmacol. 2021 Jan;11:2472 WB,IHC ; Rat.

PubMed:33584272

[IF=3.361] Ding Z et al. The CRD of Frizzled 7 exhibits chondroprotective effects in osteoarthritis via inhibition of the canonical Wnt3a/β-catenin signaling pathway. Int Immunopharmacol. 2020 Mar 6;82:106367. IHC,WB ; human&mouse.

PubMed:32151961

[IF=2.454] Dobzanski A et al. Nasal polyp fibroblasts modulate epithelial characteristics via Wnt signaling. Int Forum Allergy Rhinol. 2018 Dec;8(12):1412-1420. ICF ; Human.

PubMed:30118173

[IF=2.47] Zhu, Afang, et al. "Suppression of Wnt5a, but not Wnts, relieves chronic post-thoracotomy pain via anti-inflammatory modulation in rats." Biochemical and Biophysical Research Communications (2017). WB ; Rat.

PubMed:28870803

[IF=1.43] Jin, Xinhao, et al. "Environmental Enrichment Improves Spatial Learning and Memory in Vascular Dementia Rats with Activation of Wnt/β-Catenin Signal Pathway." Medical science monitor: international medical journal of experimental and clinical research 23 (2017): 207. WB ; Rat.

PubMed:28082734

[IF=13.12] Böscke, Robert, et al. "Wnt Signaling in Chronic Rhinosinusitis with Nasal Polyps." American Journal of Respiratory Cell and Molecular Biology ja (2017). IF(ICC) ; Human.

PubMed:28059551

[IF=1.03] Lu, Juan, et al. "Expression of Wnt3a, Wnt10b, β-catenin and DKK1 in periodontium during orthodontic tooth movement in rats." Acta Odontologica Scandinavica (2015): 1-7. IHSLCP ; Rat.

PubMed:26414930

[IF=1.48] Gao, Peng, et al. "Salvianolic acid B improves bone marrow-derived mesenchymal stem cell differentiation into alveolar epithelial cells type I via Wnt signaling." Molecular Medicine Reports. other ;

PubMed:25892295