Sample:
A431 (Human) Lysate at 30 ug
Primary: Anti- Phospho-EGFR (Ser991) (SL3104R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 130 kD
Observed band size: 130 kD
Sample:
Hela (Human) Lysate at 30 ug
Primary: Anti- Phospho-EGFR (Ser991) (SL3104R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 130 kD
Observed band size: 130 kD
Sample:
293T (Human) Lysate at 30 ug
Primary: Anti- Phospho-EGFR (Ser991) (SL3104R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 130 kD
Observed band size: 130 kD
Blank control (blue line): A431(fixed with 2% paraformaldehyde
(10 min),then permeabilized with 90% ice-cold methanol for 30 min on ice).
Primary Antibody (green line): Rabbit Anti-Phospho-EGFR (Ser991)antibody (SL3104R),Dilution: 1μg /10^6 cells.
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC,Dilution: 1μg /test.
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