Rabbit Anti-MYL3 antibody | Sunlong Medical

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Cardiac myosin light chain 1; Slow skeletal Myosin; CMH8; CMLC1; MLC1SB; MLC1V; MYL3; MYL3_HUMAN; Myosin light chain 1; Myosin light chain 1 slow twitch muscle B ventricular isoform; myosin light chain 3 alkali ventricular skeletal slow; Myosin

Cat:

SL248R

Species Reactivity：

Mouse,(predicted: Human,Rat,Chicken,Dog,Pig,Cow,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human MLC/Myosin light chain 3:101-195/195

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:1000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Muscle (Mouse) Lysate at 40 ugPrimary: Anti-MYL3 (SL248R) at 1/500 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 22 kDObserved band size: 22 kDSample: Placenta (Mouse) Lysate at 30 ugPrimary: Anti- MYL3(SL248R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Mouse IgG at 1/10000 dilutionPredicted band size: 22kDObserved band size: 22 kDTissue/cell: mouse heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-MYL3 Polyclonal Antibody, Unconjugated(SL248R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingTissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-MYL3 Polyclonal Antibody, Unconjugated(SL248R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Myosin is the major component of thick muscle filaments, and is a long asymmetric molecule containing a globular head and a long tail. The molecule consists of two heavy chains each ~200,000 daltons, and four light chains each ~16,000 - 21,000 daltons. Activation of smooth and cardiac muscle primarily involves pathways which increase calcium and myosin phosphorylation resulting in contraction. Myosin light chain phosphatase acts to regulate muscle contraction by dephosphorylating activated myosin light chain. Human myosin light chain has clinical application as a cardiac marker.

Function:
Regulatory light chain of myosin. Does not bind calcium.

Subunit:
Myosin is a hexamer of 2 heavy chains and 4 light chains.

Post-translational modifications:
The N-terminus is blocked.
N-terminus is methylated by METTL11A/NTM1.

DISEASE:
Cardiomyopathy, familial hypertrophic 8 (CMH8)[MIM:608751]: A hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death. Rarely, patients present a variant of familial hypertrophic cardiomyopathy, characterized by mid-left ventricular chamber thickening. Note=The disease is caused by mutations affecting the gene represented in this entry.

Similarity:
Contains 3 EF-hand domains.

SWISS:
P08590

Gene ID:
4634

Database links:

Entrez Gene: 4634 Human

Entrez Gene: 17897 Mouse

Entrez Gene: 24585 Rat

Omim: 160790 Human

SwissProt: P08590 Human

SwissProt: P09542 Mouse

SwissProt: P11289 Rat

Unigene: 517939 Human

Unigene: 7353 Mouse

Unigene: 1955 Rat

Picture

Sample: Placenta (Mouse) Lysate at 30 ug
Primary: Anti- MYL3(SL248R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/10000 dilution
Predicted band size: 22kD
Observed band size: 22 kD

Tissue/cell: mouse heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-MYL3 Polyclonal Antibody, Unconjugated(SL248R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-MYL3 Polyclonal Antibody, Unconjugated(SL248R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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