Rabbit Anti-Phospho-TH (Ser31) antibody

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Tyrosine Hydroxylase (Phospho-Ser31); Dystonia 14; DYT14; DYT5b; EC 1.14.16.2; OTTHUMP00000011225; OTTHUMP00000011226; ple; Protein Pale; TH; The; TY3H_RAT; TYH; Tyrosine 3 hydroxylase; Tyrosine 3 monooxygenase; Tyrosine 3-hydroxylase; Tyrosine 3-monooxyg

Cat:

SL3463R

Species Reactivity：

Mouse,Rat,

Immunogen：

KLH conjugated synthesised phosphopeptide derived from rat TH around the phosphorylation site of Ser31:VT(p-S)PR

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Lane 1: Adrenal gland (Mouse) Lysate at 40 ugLane 2: Adrenal gland (Rat) Lysate at 40 ugPrimary: Anti-Phospho-TH (Ser31) (SL3463R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 59 kDObserved band size: 59 kDParaformaldehyde-fixed, paraffin embedded (Mouse adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-TH (Ser31)) Polyclonal Antibody, Unconjugated (SL3463R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-TH (Ser31)) Polyclonal Antibody, Unconjugated (SL3463R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.Paraformaldehyde-fixed, paraffin embedded (Rat adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-TH (Ser31)) Polyclonal Antibody, Unconjugated (SL3463R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-TH (Ser31)) Polyclonal Antibody, Unconjugated (SL3463R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.

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Unit:

Price: $256.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Tyrosine hydroxylase is involved in the conversion of tyrosine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons. Tyrosine hydroxylase is regularly used as a marker for dopaminergic neurons, which is particularly relevant for research into Parkinson's disease.

Function:
Plays an important role in the physiology of adrenergic neurons.

Subunit:
Homotetramer.

Subcellular Location:
Mainly expressed in the brain and adrenal glands.

Post-translational modifications:
In vitro, phosphorylation of Ser-19 increases the rate of Ser-40 phosphorylation, which results in enzyme opening and activation.

Similarity:
Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.

SWISS:
P04177

Gene ID:
25085

Database links:

Entrez Gene: 7054 Human

Entrez Gene: 21823 Mouse

Entrez Gene: 25085 Rat

Omim: 191290 Human

SwissProt: P07101 Human

SwissProt: P24529 Mouse

SwissProt: P04177 Rat

Unigene: 435609 Human

Unigene: 1292 Mouse

Unigene: 11082 Rat

神经细胞标志物
酪氨酸羟化酶（TH）是儿茶酚胺类神经递质即多巴胺、去甲肾上腺素、肾上腺素生物合成过程所需的限速酶，它以四氢生物喋呤啶(SH4)为辅酶，催化酪氨酸的羟化而生成多巴(DOPA)。
已知在患帕金森病(Parkinson disease,PD)时，脑内多巴胺(dopamine,DA)的减少与此酶活性低下有关。因此对PD模型动物来说，若将TH基因植入脑内，便可以提高脑内DA水平而达到基因治疗目的。 Picture

Paraformaldehyde-fixed, paraffin embedded (Mouse adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-TH (Ser31)) Polyclonal Antibody, Unconjugated (SL3463R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-TH (Ser31)) Polyclonal Antibody, Unconjugated (SL3463R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-TH (Ser31)) Polyclonal Antibody, Unconjugated (SL3463R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-TH (Ser31)) Polyclonal Antibody, Unconjugated (SL3463R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.

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