Talin, a multifunctional constituent of cell-substratum attachment sites, is a high molecular weight protein (225-270 kDa) found in variety of tissues and cell types. It is localized at a subset of adherens junctions, specialized cell-cell and cell-matrix associations that are characterized by the presence of filamentous actin at the cytoplasmic face of the junctional complex. In cultured cells, talin is absent from cell-cell junctions and found predominantly at adhesion plaques and in fibrillar streaks underlying cell surface fibronectin. Talin interacts with at least two other proteins that are localized at adhesion plaques, vinculin and integrin. Talin and vinculin have been shown to interact with each other and both have been proposed to be involved in generating the transmembrane connection, between the extracellular matrix and the cytoskeleton, that occurs at adhesion plaques. At physiological ionic strength, talin is an elongate, flexible, monomeric protein with the ability to self-associate into dimers at higher protein concentrations.
Function:
Probably involved in connections of major cytoskeletal structures to the plasma membrane. High molecular weight cytoskeletal protein concentrated at regions of cell-substratum contact and, in lymphocytes, at cell-cell contacts.
Subunit:
Binds with high affinity to VCL and with low affinity to integrins. Interacts with PTK2/FAK1 (By similarity). Interacts with PIP5K1C and NRAP. Interacts with LAYN. Interacts with SYNM.
Subcellular Location:
Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytoskeleton. Note=Colocalizes with LAYN at the membrane ruffles.
Similarity:
FERM domain.
Contains 1 I/LWEQ domain.
SWISS:
Q9Y490
Gene ID:
7094
Database links:
Entrez Gene: 395194 Chicken
Entrez Gene: 7094 Human
Entrez Gene: 21894 Mouse
Entrez Gene: 313494 Rat
Omim: 186745 Human
SwissProt: P54939 Chicken
SwissProt: Q9Y490 Human
SwissProt: P26039 Mouse
Unigene: 471014 Human
Unigene: 208601 Mouse
Unigene: 7715 Rat
TALIN-踝蛋白。是重要的细胞骨架肌动蛋白结合蛋白，由两个270kDa的亚单位组成的反向
平行的同源二聚体，与整合蛋白B尾部的结合诱导了整合蛋白胞外结构域构象的改变,起到了对整合蛋白的激活。
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Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Talin) Polyclonal Antibody, Unconjugated (SL3619R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Talin) Polyclonal Antibody, Unconjugated (SL3619R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: mouse fetal liver;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-Talin Polyclonal Antibody, FITC conjugated(SL3619R-FITC) 1:200, at 1:200 dilution for 60 minutes at 37°C. DAPI(5ug/ml,blue,SLC0033) was used to stain the cell nuclei
Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-Talin Polyclonal Antibody, FITC conjugated(SL3619R-FITC) 1:200, dilution for 60 minutes at 37°C. DAPI(5ug/ml,blue,SLC0033) was used to stain the cell nuclei
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