Rabbit Anti-PFKFB2/PFK2 antibody

Home > Product > Antibody > Rabbit Anti-PFKFB2/PFK2 antibody

6 phosphofructo 2 kinase/fructose 2 6 biphosphatase 2; 6-bisphosphatase; 6-P2ase 2; 6-P2ASE heart-type isozyme; 6PF 2 K/Fru 2 6 P2ASE heart type isozyme; 6PF-2-K/Fru-2; F262_HUMAN; Fructose 2 6 bisphosphatase cardiac isozyme; Fructose-2; PFK 2/FBPase 2; P

Cat:

SL5005R

Species Reactivity：

Human,Mouse,Rat,(predicted: Chicken,Dog,)

Immunogen：

KLH conjugated synthetic peptide derived from human PFKFB2:51-150/505

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: PSLC3 Cell Lysate at 40 ug HepG2 Cell Lysate at 40 ug Primary: Anti- PFKFB2/PFK2 (SL5005R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 58 kD Observed band size: 62 kD Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PFKFB2) Polyclonal Antibody, Unconjugated (SL5005R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Tissue/cell: Rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-PFKFB2 Polyclonal Antibody, Unconjugated(SL5005R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingTissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-PFKFB2 Polyclonal Antibody, Unconjugated(SL5005R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

prev next

Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
Feedback Wall

PFKFB2 is involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. It has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2,6-bisphosphate, and a fructose-2,6-biphosphatase activity that catalyzes the degradation of fructose-2,6-bisphosphate. PFKFB2 regulates fructose-2,6-bisphosphate levels in the heart, while a related enzyme encoded by a different gene regulates fructose-2,6-bisphosphate levels in the liver and muscle. This enzyme functions as a homodimer. There are two named isoforms.

Function:
Synthesis and degradation of fructose 2,6-bisphosphate.

Subunit:
Homodimer (By similarity).

Tissue Specificity:
Heart.

Post-translational modifications:
Phosphorylation by AMPK stimulates activity.

Similarity:
In the SLCterminal section; belongs to the phosphoglycerate mutase family.

SWISS:
O60825

Gene ID:
5208

Database links:

Entrez Gene: 5208 Human

Omim: 171835 Human

SwissProt: O60825 Human

Picture

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PFKFB2) Polyclonal Antibody, Unconjugated (SL5005R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Tissue/cell: Rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-PFKFB2 Polyclonal Antibody, Unconjugated(SL5005R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-PFKFB2 Polyclonal Antibody, Unconjugated(SL5005R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Product Feedback Wall