Rabbit Anti-SLC39A7 antibody

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D6S115E; D6S2244E; H2 KE4; Histidine rich membrane protein Ke4; Histidine-rich membrane protein Ke4; HKE4; HLA class II region expressed gene KE4; KE4; Ke4 gene mouse, human homolog of; Really interesting new gene 5 protein; RING5; S39A7_HUMAN; SLC39A7; S

Cat:

SL2391R

Species Reactivity：

Mouse,Rat,(predicted: Human,Dog,Pig,Horse,Rabbit,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human SLC39A7:201-300/469

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Lung (Mouse) Lysate at 40 ugPrimary: Anti-SLC39A7 (SL2391R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 50 kDObserved band size: 50 kDSample: Cerebrum (Mouse) Lysate at 40 ugPrimary: Anti-C39A7 (SL2391R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 50 kDObserved band size: 50 kDParaformaldehyde-fixed, paraffin embedded (mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SLC39A7) Polyclonal Antibody, Unconjugated (SL2391R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SLC39A7) Polyclonal Antibody, Unconjugated (SL2391R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Zinc is an essential cofactor for more than 50 classes of enzymes and is involved in protein, nucleic acid, carbohydrate, and lipid metabolism, plus the control of gene transcription, growth, development, and differentiation. Zinc cannot passively diffuse across cell membranes and requires specific transporters, such as SLC39A7, to enter the cytosol from both the extracellular environment and from intracellular storage compartments.

Subcellular Location:
Membrane; Multi-pass membrane protein (Probable).

Tissue Specificity:
Major expression in placenta, lung, kidney and pancreas.

Similarity:
Belongs to the ZIP transporter (TC 2.A.5) family. KE4/Catsup subfamily.

SWISS:
Q92504

Gene ID:
7922

Database links:

Entrez Gene: 7922 Human

Entrez Gene: 14977 Mouse

Entrez Gene: 294281 Rat

Omim: 601416 Human

SwissProt: Q92504 Human

SwissProt: Q31125 Mouse

Unigene: 631995 Human

Unigene: 18556 Mouse

Picture

Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti-C39A7 (SL2391R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD

Paraformaldehyde-fixed, paraffin embedded (mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SLC39A7) Polyclonal Antibody, Unconjugated (SL2391R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SLC39A7) Polyclonal Antibody, Unconjugated (SL2391R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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