Rabbit Anti-Phospho-FGFR1+FGFR2 (Tyr463/Tyr466) antibody

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FGFR1 + FGFR2 (Phospho Tyr463/Tyr466); FGFR1 + FGFR2 (Phospho Tyr463+Tyr466); FGFR2 (Phospho Y463+Y466); bFGF R; BFGFR; C FGR; CD 331; CD331; CD331 antigen; CEK; FGFBR; FGFR 1; Fibroblast growth factor receptor 1; FLG; FLG protein; FLJ14326; FLT 2; FLT2;

Cat:

SL5326R

Species Reactivity：

Human,Rat,(predicted: Mouse,Chicken,Pig,Cow,Horse,Rabbit,)

Immunogen：

KLH conjugated Synthesised phosphopeptide derived from human FGFR1 around the phosphorylation site of Tyr463:SE(p-Y)EL<Cytoplasmic>

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: U87MG(Human) Cell Lysate at 30 ugPrimary: Anti-Phospho-FGFR1+FGFR2 (Tyr463/Tyr466) (SL5326R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 88 kDObserved band size: 73 kDParaformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-FGFR1+FGFR2 (Tyr463/Tyr466)) Polyclonal Antibody, Unconjugated (SL5326R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (human laryngeal carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-FGFR1+FGFR2 (Tyr463/Tyr466)) Polyclonal Antibody, Unconjugated (SL5326R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-FGFR1+FGFR2 (Tyr463/Tyr466)) Polyclonal Antibody, Unconjugated (SL5326R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (Human glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-FGFR1+FGFR2 (Tyr463 Tyr466)) Polyclonal Antibody, Unconjugated (SL5326R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-FGFR1+FGFR2 (Tyr463 Tyr466)) Polyclonal Antibody, Unconjugated (SL5326R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Antigen:1.phosphopeptide(blue),0.2ug/100ul;2.non-phosphopeptide(red),0.2ug/100ul;Primary:Antibody,1:200,1:400,1:800,1:1600,1:640,1:1280,1:2560;Secondary:HRP conjugated Goat Anti-Rabbit igG(BSL0295G-HRP)at1:5000;TMB(SLC0024)staining:Read the data in Microplate Reader by 450nm

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Unit:

Price: $256.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Fibroblast growth factors (FGFs) produce mitogenic and angiogenic effects in target cells by signaling through the cellular surface tyrosine kinase receptors. There are four members of the FGF receptor family: FGFR-1 (flg), FGFR-2 (bek, KGFR), FGFR-3 and FGFR-4. Each receptor contains an extracellular ligand binding domain, a transmembrane region and a cytoplasmic kinase domain (1). Following ligand binding and dimerization, the receptors are phosphorylated at specific tyrosine residues (2). Seven tyrosine residues in the cytoplasmic tail of FGFR-1 can be phosphorylated: Tyr463, Tyr583, Tyr585, Tyr653, Tyr654, Tyr730 and Tyr766. Tyrosine 653 and 654 are important for catalytic activity of the activated FGFR and are essential for signaling (3). The other phosphorylated tyrosine residues may provide docking sites for downstream signaling components such as Crk and PLCgamma.

Function:
Receptor for fibroblast growth factors FGF2 and FGF1. Receptor for FGF23 in the presence of KL (By similarity). Promotes mitogenesis in response to fibroblast growth factors. Activates PLCG1.

Subunit:
Homodimer. Interacts with KLB. Interacts with KL and FGF23. Interacts with SHB and GRB10. Interacts with PLCG1 (via SH2 domains). Interacts with KAL1; this interaction does not interfere with FGF2-binding to FGFR1, but prevents binding of heparin-bound FGF2. Interacts with SOX2 and SOX3.

Subcellular Location:
Membrane; Single-pass type I membrane protein.

Tissue Specificity:
Detected in astrocytoma, neuroblastoma and adrenal cortex cell lines. Some isoforms are detected in foreskin fibroblast cell lines, however isoform 17, isoform 18 and isoform 19 are not detected in these cells.

Post-translational modifications:
Binding of FGF1 and heparin promotes autophosphorylation on tyrosine residues and activation of the receptor.

DISEASE:
Defects in FGFR1 are a cause of Pfeiffer syndrome (PS) [MIM:101600]; also known as acrocephalosyndactyly type V (ACS5). PS is characterized by craniosynostosis (premature fusion of the skull sutures) with deviation and enlargement of the thumbs and great toes, brachymesophalangy, with phalangeal ankylosis and a varying degree of soft tissue syndactyly.
Defects in FGFR1 are a cause of idiopathic hypogonadotropic hypogonadism (IHH) [MIM:146110]. IHH is defined as a deficiency of the pituitary secretion of follicle-stimulating hormone and luteinizing hormone, which results in the impairment of pubertal maturation and of reproductive function.

Similarity:
Belongs to the protein kinase superfamily. Tyr protein kinase family. Fibroblast growth factor receptor subfamily.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain.

SWISS:
P11362

Gene ID:
2260

Database links:

Entrez Gene: 2260 Human

Entrez Gene: 14182 Mouse

Entrez Gene: 79114 Rat

Omim: 136350 Human

SwissProt: P11362 Human

SwissProt: P16092 Mouse

SwissProt: Q04589 Rat

Unigene: 264887 Human

Unigene: 265716 Mouse

Unigene: 207203 Rat

Unigene: 9797 Rat

碱性成纤维细胞生长因子(bFGF)是一种多功能的生长因子,具有促进细胞有丝分裂和诱导新血管形成作用,和其受体(FGFR1)碱性成纤维细胞生长因子受体结合之后,在人体各组织中具有广泛的生物学活性和生理病理作用.越来越多的研究表明肿瘤细胞中bFGF-R1过度表达对肿瘤发生发展具有重要意义。 Picture

Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-FGFR1+FGFR2 (Tyr463/Tyr466)) Polyclonal Antibody, Unconjugated (SL5326R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human laryngeal carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-FGFR1+FGFR2 (Tyr463/Tyr466)) Polyclonal Antibody, Unconjugated (SL5326R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-FGFR1+FGFR2 (Tyr463/Tyr466)) Polyclonal Antibody, Unconjugated (SL5326R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-FGFR1+FGFR2 (Tyr463 Tyr466)) Polyclonal Antibody, Unconjugated (SL5326R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-FGFR1+FGFR2 (Tyr463 Tyr466)) Polyclonal Antibody, Unconjugated (SL5326R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Antigen: 1.phosphopeptide(blue),0.2ug/100ul; 2.non-phosphopeptide(red),0.2ug/100ul; Primary:Antibody,1:200,1:400,1:800,1:1600,1:640,1:1280,1:2560; Secondary:HRP conjugated Goat Anti-Rabbit igG(BSL0295G-HRP)at1:5000; TMB(SLC0024)staining: Read the data in Microplate Reader by 450nm

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