Rabbit Anti-phospho-MEF2C (Ser396) antibody

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MEF2C (phospho S396); p-MEF2C (phospho S396); MEF2C(phospho S396); MADS box transcription enhancer factor 2 polypeptide C (myocyte enhancer factor 2C); Myocyte enhancer factor 2C; Myocyte specific enhancer factor 2C; Similar to MADS box transcription enha

Cat:

SL596R

Species Reactivity：

Mouse,Rat,(predicted: Human,Dog,Pig,Rabbit,)

Immunogen：

KLH conjugated Synthesised phosphopeptide derived from human MEF2C around the phosphorylation site of Ser396:PV(p-S)PP

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Muscle (Mouse)Lysate at 40 ugPrimary: Anti-p-MEF2C(Ser396)(SL596R)at 1/300 dilutionSecondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilutionPredicted band size: 51kDObserved band size: 51kD Sample:Kidney (Rat)Lysate at 40 ugPrimary: Anti-p-MEF2C(Ser396)(SL596R)at 1/300 dilutionSecondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilutionPredicted band size: 51kDObserved band size: 51kDSample: Cerebrum(Mouse)Lysate at 40 ugPrimary: Anti-p-MEF2C(Ser396)(SL596R)at 1/300 dilutionSecondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilutionPredicted band size: 51kDObserved band size: 51kDSample: Lymph node(Mouse)Lysate at 40 ugPrimary: Anti-p-MEF2C(Ser396)(SL596R)at 1/300 dilutionSecondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilutionPredicted band size: 51kDObserved band size: 51kD

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Unit:

Price: $256.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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MEF2C is a transcription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. This protein controls cardiac morphogenesis and myogenesis, and is also involved in vascular development. It may also be involved in neurogenesis and in the development of cortical architecture.

Function:
Transcription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. Controls cardiac morphogenesis and myogenesis, and is also involved in vascular development. Plays an essential role in hippocampal-dependent learning and memory by suppressing the number of excitatory synapses and thus regulating basal and evoked synaptic transmission. Crucial for normal neuronal development, distribution, and electrical activity in the neocortex. Necessary for proper development of megakaryocytes and platelets and for bone marrow B lymphopoiesis. Required for B-cell survival and proliferation in response to BCR stimulation, efficient IgG1 antibody responses to T-cell-dependent antigens and for normal induction of germinal center B cells. May also be involved in neurogenesis and in the development of cortical architecture (By similarity). Isoform 3 and isoform 4, which lack the repressor domain, are more active than isoform 1 and isoform 2.

Subunit:
Forms a complex with class II HDACs in undifferentiating cells. On myogenic differentiation, HDACs are released into the cytoplasm allowing MEF2s to interact with other proteins for activation. Interacts with EP300 in differentiating cells; the interaction acetylates MEF2C leading to increased DNA binding and activation. Interacts with HDAC7 and CARM1 (By similarity). Interacts with HDAC4, HDAC7 AND HDAC9; the interaction WITH HDACs represses transcriptional activity. Interacts with MYOCD (By similarity).

Subcellular Location:
Nucleus.

Tissue Specificity:
Expressed in brain and skeletal muscle.

Post-translational modifications:
Phosphorylation on Ser-59 enhances DNA binding activity (By similarity). Phosphorylation on Ser-396 is required for Lys-391 sumoylation and inhibits transcriptional activity.
Acetylated by p300 on several sites in diffentiating myocytes. Acetylation on Lys-4 increases DNA binding and transactivation.
Sumoylated on Lys-391 with SUMO2 but not by SUMO1 represses transcriptional activity.
Proteolytically cleaved in cerebellar granule neurons, probably by caspase 7, following neurotoxicity. Preferentially cleaves the CDK5-mediated hyperphosphorylated form which leads to neuron apoptosis and transcriptional inactivation.

DISEASE:
Mental retardation, autosomal dominant 20 (MRD20) [MIM:613443]: A disorder characterized by severe mental retardation, absent speech, hypotonia, poor eye contact and stereotypic movements. Dysmorphic features include high broad forehead with variable small chin, short nose with anteverted nares, large open mouth, upslanted palpebral fissures and prominent eyebrows. Some patients have seizures. Note=The disease is caused by mutations affecting the gene represented in this entry.

Similarity:
Belongs to the MEF2 family.
Contains 1 MADS-box domain.
Contains 1 Mef2-type DNA-binding domain.

SWISS:
Q06413

Gene ID:
4208

Database links:

Entrez Gene: 512254 Cow

Entrez Gene: 4208 Human

Entrez Gene: 17260 Mouse

Entrez Gene: 733590 Pig

Entrez Gene: 499497 Rat

Omim: 600662 Human

SwissProt: Q2KIA0 Cow

SwissProt: Q06413 Human

SwissProt: Q8CFN5 Mouse

SwissProt: A4UTP7 Pig

Unigene: 649965 Human

Unigene: 4801 Mouse

Unigene: 451574 Mouse

Unigene: 416898 Mouse

Picture

Sample:Kidney (Rat)Lysate at 40 ug
Primary: Anti-p-MEF2C(Ser396)(SL596R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 51kD
Observed band size: 51kD

Sample: Cerebrum(Mouse)Lysate at 40 ug
Primary: Anti-p-MEF2C(Ser396)(SL596R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 51kD
Observed band size: 51kD

Sample: Lymph node(Mouse)Lysate at 40 ug
Primary: Anti-p-MEF2C(Ser396)(SL596R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 51kD
Observed band size: 51kD

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