Rabbit Anti-SMAD9 antibody | Sunlong Medical

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AI528653 ; BSP 1 ; BSP1 ; DKFZp781C1895 ; DKFZp781O1323 ; Dwarfin A ; Dwf A ; DwfA ; Dwfc ; hSMAD1 ; hSmad5 ; JV 41 ; JV4 1 ; JV41 ; JV5 1 ; MAD (mothers against decapentaplegic, Drosophila) homolog 1 ; MAD (mothers against decapentaplegic

Cat:

SL4253R

Species Reactivity：

Human,Mouse,Rat,(predicted: Chicken,Dog,Pig,Cow,Horse,)

Immunogen：

KLH conjugated synthetic peptide derived from human SMAD9:361-467/467

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=3μg/TestIF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Heart (Mouse) Lysate at 40 ugMuscle (Mouse) Lysate at 40 ugPrimary: Anti-SMAD9 (SL4253R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 52 kDObserved band size: 52 kDSample: A549(Human) Cell Lysate at 30 ugPrimary: Anti-SMAD9 (SL4253R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 52 kDObserved band size: 52 kDParaformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SMAD9) Polyclonal Antibody, Unconjugated (SL4253R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Positive control: Hela cellsConcebtration: 3μg/10^6 cellsIncubation conditions: Avoid light , 30 minutes on the ice.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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SMAD9 is a member of the MAD-related family of molecules. MAD-related proteins are a recently identified family of intracellular proteins that are thought to be essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta superfamily.

Function:
Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD9 is a receptor-regulated SMAD (R-SMAD).

Subunit:
Interaction with the co-SMAD SMAD4. Interacts with PEBP2-alpha subunit.

Subcellular Location:
Cytoplasm (By similarity). Nucleus (By similarity). Note=In the cytoplasm in the absence of ligand. Migration to the nucleus when complexed with SMAD4 (By similarity).

Tissue Specificity:
Expressed in heart, brain, placenta, lung, skeletal muscle, prostate, testis, ovary and small intestine. Also expressed in fetal brain, lung and kidney.

Post-translational modifications:
Phosphorylated on serine by BMP (bone morphogenetic proteins) type 1 receptor kinase.

DISEASE:
Defects in SMAD9 may be a cause of primary pulmonary hypertension (PPH1) [MIM:178600]. A rare disorder characterized by plexiform lesions of proliferating endothelial cells in pulmonary arterioles. The lesions lead to elevated pulmonary arterial pression, right ventricular failure, and death. The disease can occur from infancy throughout life and it has a mean age at onset of 36 years. Penetrance is reduced. Although familial PPH1 is rare, cases secondary to known etiologies are more common and include those associated with the appetite-suppressant drugs.

Similarity:
Belongs to the dwarfin/SMAD family.
Contains 1 MH1 (MAD homology 1) domain.
Contains 1 MH2 (MAD homology 2) domain.

SWISS:
O15198

Gene ID:
4093

Database links:

Entrez Gene: 4093 Human

Entrez Gene: 55994 Mouse

Entrez Gene: 85435 Rat

Omim: 603295 Human

SwissProt: O15198 Human

SwissProt: Q9JIW5 Mouse

SwissProt: O54835 Rat

Unigene: 123119 Human

Unigene: 244353 Mouse

Unigene: 10862 Rat

Picture

Sample:
A549(Human) Cell Lysate at 30 ug
Primary: Anti-SMAD9 (SL4253R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kD
Observed band size: 52 kD

Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SMAD9) Polyclonal Antibody, Unconjugated (SL4253R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Positive control: Hela cells
Concebtration: 3μg/10^6 cells
Incubation conditions: Avoid light , 30 minutes on the ice.

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