Rabbit Anti-CCDC106 antibody

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Ccdc106; Coiled-coil domain-containing protein 106; Protein predicted by clone 23882; ZNF581; CC106_HUMAN.

Cat:

SL5711R

Species Reactivity：

Human,Mouse,Rat,(predicted: Dog,Cow,Horse,)

Immunogen：

KLH conjugated synthetic peptide derived from human CCDC106:201-280/280

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Jurkat(Human) Cell Lysate at 30 ugPrimary: Anti- CCDC106 (SL5711R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 31 kDObserved band size: 31 kDSample: A549(Human) Cell Lysate at 30 ugPrimary: Anti- CCDC106 (SL5711R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 31 kDObserved band size: 31 kDParaformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CCDC106) Polyclonal Antibody, Unconjugated (SL5711R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CCDC106) Polyclonal Antibody, Unconjugated (SL5711R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Promotes the degradation of p53/TP53 protein and inhibits its transactivity.

Function:
Promotes the degradation of p53/TP53 protein and inhibits its transactivity.

Subunit:
Interacts with p53/TP53.

Subcellular Location:
Nucleus. Note=Colocalizes with p53/TP53.

SWISS:
Q9BWC9

Gene ID:
29903

Database links:

Entrez Gene: 29903 Human

Entrez Gene: 232821 Mouse

Entrez Gene: 499071 Rat

Omim: 613478 Human

SwissProt: Q9BWC9 Human

SwissProt: Q3ULM0 Mouse

Unigene: 82482 Human

Unigene: 482157 Mouse

Unigene: 488775 Mouse

CCDC106通过与p53蛋白相互作用，促进p53蛋白的降解，导致p53功能失活。CCDC106基因很可能是一个癌基因，CCDC106的高表达可能是导致具有野生型p53肿瘤发生的原因之一。 Picture

Sample:
A549(Human) Cell Lysate at 30 ug
Primary: Anti- CCDC106 (SL5711R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31 kD
Observed band size: 31 kD

Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CCDC106) Polyclonal Antibody, Unconjugated (SL5711R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CCDC106) Polyclonal Antibody, Unconjugated (SL5711R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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