SIRT7 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family.
Function:
NAD-dependent protein deacetylase. Required to restore the transcription of ribosomal RNA (rRNA) at the exit from mitosis. Promotes the association of RNA polymerase I with the rDNA promoter region and coding region. Stimulates transcription activity of the RNA polymerase I complex.
Subunit:
Interacts with UBTF and the RNA polymerase I complex. Interacts with histone H2A and/or histone H2B.
Subcellular Location:
Cytoplasm. Nucleus, nucleolus. Note=Located close to the nuclear membrane when in the cytoplasm. Associated with chromatin. Associated with rDNA promoter and transcribed region. Associated with nucleolar organizer regions during mitosis.
Post-translational modifications:
Phosphorylated during mitosis (Probable).
Similarity:
Belongs to the sirtuin family. Class IV subfamily.
Contains 1 deacetylase sirtuin-type domain.
SWISS:
Q9NRC8
Gene ID:
51547
Database links:
Entrez Gene: 505662 Cow
Entrez Gene: 51547 Human
Entrez Gene: 209011 Mouse
Omim: 606212 Human
SwissProt: Q0P595 Cow
SwissProt: Q9NRC8 Human
SwissProt: Q8BKJ9 Mouse
Unigene: 514636 Human
Unigene: 292957 Mouse
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Sample:Small intestine (Mouse)Lysate at 40 ug
Primary: Anti-SIRT7(SL5973R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 45kD
Observed band size: 50kD
Sample:Lymph node (Mouse)Lysate at 40 ug
Primary: Anti-SIRT7(SL5973R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 45kD
Observed band size: 50kD
Tissue/cell: mouse placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-SIRT7 Polyclonal Antibody, Unconjugated(SL5973R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
Blank control: mouse splenocytes(blue)
Isotype Control Antibody: Rabbit IgG(orange) ;
Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue),
Dilution: 1:100 in 1 X PBS containing 0.5% BSA ;
Primary Antibody Dilution: 1μl in 100 μL1X PBS containing 0.5% BSA(green).
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