Rabbit Anti-WNT2 antibody | Sunlong Medical

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Int 1 related protein; IRP protein; WNT 2; INT1L 1; INT1L1; ONCOGENE INT1 LIKE 1; Wingless type MMTV integration site family member 2; WNT2_HUMAN.

Cat:

SL6133R

Species Reactivity：

Human,Mouse,(predicted: Rat,Chicken,Cow,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human WNT2:221-320/360

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Brain (Mouse) Lysate at 40 ugPrimary: Anti- WNT2 (SL6133R) at 1/300 dilutionSecondary: HRP conjugated Goat-Anti-rabbit IgG (SL0295G-HRP) at 1/5000 dilutionPredicted band size: 37 kDObserved band size: 38 kDImages provided the Independent Validation Program (badge number 029629)Formalin-fixed and paraffin embedded human brain tissue with Parkinson's morphology labeled with Rabbit Anti-WNT2 Polyclonal Antibody (SL6133R) at 1:250 overnight at 4 °C followed by conjugation to secondary antibody.Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (WNT2) Polyclonal Antibody, Unconjugated (SL6133R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Images provided the Independent Validation Program (badge number 029629)Formalin-fixed and paraffin embedded human brain glioma tissue labeled with Rabbit Anti-WNT2 Polyclonal Antibody (SL6133R) at 1:250 overnight at 4 °C followed by conjugation to secondary antibody.

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis.

Subcellular Location:
Secreted.

Tissue Specificity:
Expressed in brain in the thalamus, in fetal and adult lung and in placenta.

Similarity:
Belongs to the Wnt family.

SWISS:
P09544

Gene ID:
7472

Database links:

Entrez Gene: 7472 Human

Entrez Gene: 22413 Mouse

Entrez Gene: 114487 Rat

Omim: 147870 Human

SwissProt: P09544 Human

SwissProt: P21552 Mouse

Unigene: 567356 Human

Unigene: 33653 Mouse

Picture

Images provided the Independent Validation Program (badge number 029629)Formalin-fixed and paraffin embedded human brain tissue with Parkinson's morphology labeled with Rabbit Anti-WNT2 Polyclonal Antibody (SL6133R) at 1:250 overnight at 4 °C followed by conjugation to secondary antibody.

Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (WNT2) Polyclonal Antibody, Unconjugated (SL6133R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Images provided the Independent Validation Program (badge number 029629)Formalin-fixed and paraffin embedded human brain glioma tissue labeled with Rabbit Anti-WNT2 Polyclonal Antibody (SL6133R) at 1:250 overnight at 4 °C followed by conjugation to secondary antibody.

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