Rabbit Anti-Alpha 2 antiplasmin antibody

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A2AP; A2AP_HUMAN; AAP; Alpha 2 antiplasmin; Alpha 2 antiplasmin pigment epithelium derived factor; Alpha 2 AP; ALPHA 2 PI; Alpha 2 plasmin inhibitor; Alpha 2 plasmin inhibitor deficiency; Alpha-2-antiplasmin; Alpha-2-AP; Alpha-2-PI; Alpha-2-plasmin inhibi

Cat:

SL6188R

Species Reactivity：

Human,Mouse,Rat,(predicted: Dog,Horse,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human SERPINF2:161-260/491

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Liver (Mouse) Lysate at 40 ugPrimary: Anti- Alpha 2 antiplasmin (SL6188R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 50 kDObserved band size: 52 kDParaformaldehyde-fixed, paraffin embedded (human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Alpha 2 antiplasmin) Polyclonal Antibody, Unconjugated (SL6188R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Alpha 2 antiplasmin) Polyclonal Antibody, Unconjugated (SL6188R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Alpha 2 Antiplasmin is a member of the serpin family of serine protease inhibitors. The protein is a major inhibitor of plasmin, which degrades fibrin and various other proteins. Consequently, the proper function of this gene has a major role in regulating the blood clotting pathway. Mutations in this gene result in alpha-2-plasmin inhibitor deficiency, which is characterized by severe hemorrhagic diathesis. Multiple transcript variants encoding different isoforms have been found for this gene.

Function:
Serine protease inhibitor. The major targets of this inhibitor are plasmin and trypsin, but it also inactivates matriptase-3/TMPRSS7 and chymotrypsin.

Subunit:
Forms protease inhibiting heterodimer with TMPRSS7.

Subcellular Location:
Secreted.

Tissue Specificity:
Expressed by the liver and secreted in plasma.

DISEASE:
Alpha-2-plasmin inhibitor deficiency (APLID) [MIM:262850]: An autosomal recessive disorder resulting in severe hemorrhagic diathesis. Note=The disease is caused by mutations affecting the gene represented in this entry.

Similarity:
Belongs to the serpin family.

SWISS:
P08697

Gene ID:
5345

Database links:

Entrez Gene: 5345 Human

Omim: 613168 Human

SwissProt: P08697 Human

Unigene: 159509 Human

Picture

Paraformaldehyde-fixed, paraffin embedded (human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Alpha 2 antiplasmin) Polyclonal Antibody, Unconjugated (SL6188R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Alpha 2 antiplasmin) Polyclonal Antibody, Unconjugated (SL6188R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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