Rabbit Anti-KCNK3 antibody | Sunlong Medical

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cTBAK 1; Acid sensitive potassium channel protein TASK 1; Cardiac two pore background K(+) channel; K2p3.1; OAT1; potassium channel, subfamily K, member 3; rTASK; TASK 1; TBAK1; TWIK related acid sensitive K+ channel; Two pore potassium channel KT3.1; KCN

Cat:

SL6674R

Species Reactivity：

Mouse,Rat,(predicted: Human,Chicken,Dog,Cow,Rabbit,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human KCNK3:51-150/394<Extracellular>

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Pancreas (Mouse) Lysate at 40 ugPrimary: Anti-KCNK3 (SL6674R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 43 kDObserved band size: 43 kDSample: Placenta (Mouse) Lysate at 40 ugPrimary: Anti-KCNK3 (SL6674R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 43 kDObserved band size: 43 kDSample: Urinary bladder (Mouse) Lysate at 40 ugPrimary: Anti- KCNK3 (SL6674R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 43 kDObserved band size: 49 kDBlank control(blue): H9C2 (fixed with 2% paraformaldehyde (10 min)) . Primary Antibody:Rabbit Anti- KCNK3 antibody(SL6674R), Dilution: 6ug in 100 uL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This gene encodes a member of the superfamily of potassium channel proteins that contain two pore-forming P domains. The encoded protein is an outwardly rectifying channel that is sensitive to changes in extracellular pH and is inhibited by extracellular acidification. Also referred to as an acid-sensitive potassium channel, it is activated by the anesthetics halothane and isoflurane. Although three transcripts are detected in northern blots, there is currently no sequence available to confirm transcript variants for this gene. [provided by RefSeq, Aug 2008].

Function:
pH-dependent, voltage-insensitive, background potassium channel protein. Rectification direction results from potassium ion concentration on either side of the membrane. Acts as an outward rectifier when external potassium concentration is low. When external potassium concentration is high, current is inward.

Subcellular Location:
Membrane; Multi-pass membrane protein (Potential).

Tissue Specificity:
Widespread expression in adult. Strongest expression in pancreas and placenta. Lower expression in brain, lung, prostate, heart, kidney, uterus, small intestine and colon.

Similarity:
Belongs to the two pore domain potassium channel (TC 1.A.1.8) family.

SWISS:
O14649

Gene ID:
3777

Database links:

Entrez Gene: 3777 Human

Entrez Gene: 16527 Mouse

Entrez Gene: 29553 Rat

Omim: 603220 Human

SwissProt: O14649 Human

SwissProt: Q53SU2 Human

SwissProt: O35111 Mouse

SwissProt: O54912 Rat

Unigene: 645288 Human

Unigene: 439936 Mouse

Unigene: 15536 Rat

Picture

Sample: Placenta (Mouse) Lysate at 40 ug
Primary: Anti-KCNK3 (SL6674R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43 kD
Observed band size: 43 kD

Sample:
Urinary bladder (Mouse) Lysate at 40 ug
Primary: Anti- KCNK3 (SL6674R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43 kD
Observed band size: 49 kD

Blank control(blue): H9C2 (fixed with 2% paraformaldehyde (10 min)) .
Primary Antibody:Rabbit Anti- KCNK3 antibody(SL6674R), Dilution: 6ug in 100 uL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

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