Rabbit Anti-C3a Receptor antibody

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AZ 3B; AZ3B; C3a anaphylatoxin chemotactic receptor; C3A R; C3A R1; C3a-R; C3AR; C3AR_HUMAN; C3AR1; C3R1; Complement component 3 receptor 1; Complement component 3a receptor 1; HNFAG09.

Cat:

SL2955R

Species Reactivity：

Human,Mouse,(predicted: Rat,Horse,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human C3a Receptor/C3aR:401-482/482<Cytoplasmic>

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: K562(Human) Cell Lysate at 30 ug293T(Human) Cell Lysate at 30 ugA549(Human) Cell Lysate at 30 ugLung (Mouse) Lysate at 40 ugPrimary: Anti-C3a Receptor (SL2955R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 54 kDObserved band size: 64 kDSample: 293T Cell (Human) Lysate at 30 ugPrimary: Anti-C3a Receptor (SL2955R)at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 54kDObserved band size: 60kDSample: A549 Cell (Human) Lysate at 30 ugPrimary: Anti-C3a Receptor (SL2955R)at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 54kDObserved band size: 60kDSample: Lane 1: Rat Spleen tissue lysates Lane 2: Human A549 cell lysatesLane 3: Human HL60 cell lysates Lane 4: Human THP-1 cell lysates Primary: Anti-C3a Receptor (SL2955R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 54 kDaObserved band size: 54 kDaParaformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (C3a Receptor) Polyclonal Antibody, Unconjugated (SL2955R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Complement C3 precursor contains complement C3 beta chain, complement C3 alpha chain, C3a anaphylatoxin, complement C3b alpha chain, complement C3c fragment, complement C3dg fragment, complement C3g fragment, complement C3d fragment and complement C3f fragment. C3a, C4a, and C5a are potent anaphylatoxins that are released during complement activation, a system of ligand-surface protein interactions specific to cells of hematopoietic lineage that aids in the elimination of pathogens. C3a and C5a secretion correlates with pathophysiological phenotypes such as asthma and bacterial meningitis. Binding of these proteins to their respective G protein-coupled receptors (C3aR, C5aR), which are present on the surface of myeloid leukocytes, induces proinflammatory events such as cellular degranulation, smooth muscle contraction, arachidonic acid metabolism, cytokine release, leukocyte activation and cellular chemotaxis. C3aR is expressed in brain and activated B-lymphocytes whereas C5aR is prevalent on the surface of hepatocyte, lung, smooth muscle, and endothelial cells. Upon activation, C3aR and C5aR are susceptible to rapid GRK-mediated phosphorylation and clathrin-coated vesicle targeting. C5aR utilizes the Ras-Raf-ERK1/2 cascade and couples to Gi/G16 proteins.

Function:
Receptor for the chemotactic and inflammatory peptide anaphylatoxin C3a. This receptor stimulates chemotaxis, granule enzyme release and superoxide anion production.

Subcellular Location:
Cell membrane; Multi-pass membrane protein.

Tissue Specificity:
Widely expressed in several differentiated hematopoietic cell lines, in the lung, spleen, ovary, placenta, small intestine, throughout the brain, heart, and endothelial cells. Mostly expressed in lymphoid tissues.

Post-translational modifications:
Among the sulfation sites Tyr-174 is essential for binding of C3a anaphylatoxin.

Similarity:
Belongs to the G-protein coupled receptor 1 family.

SWISS:
Q16581

Gene ID:
719

Database links:

Entrez Gene: 719 Human

Entrez Gene: 12267 Mouse

Entrez Gene: 16807 Rat

Omim: 605246 Human

SwissProt: Q16581 Human

SwissProt: O09047 Mouse

SwissProt: O55197 Rat

Unigene: 591148 Human

Unigene: 488 Mouse

Unigene: 9772 Rat

Picture

Sample: 293T Cell (Human) Lysate at 30 ug
Primary: Anti-C3a Receptor (SL2955R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54kD
Observed band size: 60kD

Sample: A549 Cell (Human) Lysate at 30 ug
Primary: Anti-C3a Receptor (SL2955R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54kD
Observed band size: 60kD

Sample:
Lane 1: Rat Spleen tissue lysates
Lane 2: Human A549 cell lysates
Lane 3: Human HL60 cell lysates
Lane 4: Human THP-1 cell lysates
Primary: Anti-C3a Receptor (SL2955R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kDa
Observed band size: 54 kDa

Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (C3a Receptor) Polyclonal Antibody, Unconjugated (SL2955R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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