Rabbit Anti-14-3-3 zeta+delta antibody

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14 3 3 protein delta; 14 3 3 protein zeta/delta; 14 3 3 protein/cytosolic phospholipase A2; 14-3-3 protein zeta/delta; 14-3-3zeta; 14-3-3 delta; 14-3-3 zeta; 14-3-3delta; 1433Z_HUMAN; KCIP 1; KCIP-1; KCIP1; MGC111427; MGC126532; MGC13815; Phospholipase A2

Cat:

SL6790R

Species Reactivity：

Human,Mouse,Rat,(predicted: Chicken,Dog,Cow,Horse,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human 14-3-3 zeta/delta:51-150/245

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000Flow-Cyt=1ug/Testnot yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Cerebrum (Mouse) Lysate at 40 ugPrimary: Anti-14-3-3 zeta+delta (SL6790R) at 1/500 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 27 kDObserved band size: 27 kDSample: Cerebrum (Rat) Lysate at 40 ugPrimary: Anti-14-3-3 zeta+delta (SL6790R) at 1/500 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 27 kDObserved band size: 27 kDBlank control (blue line): HL60 (fixed with 70% ethanol (overninght at 4℃) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature). Primary Antibody (green line): Rabbit Anti-14-3-3 zeta+delta antibody (SL6790R),Dilution: 0.2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE<, Dilution: 1μg /test. Blank control (black line): Raji (black) (The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with PBST for 30 min on room temperature) Primary Antibody (rad line): Rabbit Anti-14-3-3 zeta+delta antibody (SL6790R) ;Dilution: 1μg /10^6 cells; Isotype Control Antibody (grenn line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE;Dilution: 1μg /test.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.

Function:
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.

Subunit:
Interacts with CDK16 and BSPRY. Interacts with WEE1 (SLCterminal). Interacts with SAMSN1. Interacts with MLF1 (phosphorylated form); the interaction retains it in the cytoplasm. Interacts with Thr-phosphorylated ITGB2. Interacts with BCL2L11. Homodimer. Heterodimerizes with YWHAE. Homo- and hetero-dimerization is inhibited by phosphorylation on Ser-58. Interacts with FOXO4, NOXA1, SSH1 and ARHGEF2. Interacts with Pseudomonas aeruginosa exoS (unphosphorylated form). Interacts with BAX; the interaction occurs in the cytoplasm. Under stress conditions, MAPK8-mediated phosphorylation releases BAX to mitochondria. Interacts with phosphorylated RAF1; the interaction is inhibited when YWHAZ is phosphorylated on Thr-232. Interacts with TP53; the interaction enhances p53 transcriptional activity. The Ser-58 phosphorylated form inhibits this interaction and p53 transcriptional activity. Interacts with ABL1 (phosphorylated form); the interaction retains ABL1 in the cytoplasm. Interacts with PKA-phosphorylated AANAT; the interaction modulates AANAT enzymatic activity by increasing affinity for arylalkylamines and acetyl-CoA and protecting the enzyme from dephosphorylation and proteasomal degradation. It may also prevent thiol-dependent inactivation. Interacts with AKT1; the interaction phosphorylates YWHAZ and modulates dimerization. Interacts with GAB2 and TLK2.

Subcellular Location:
Cytoplasm. Melanosome. Note=Located to stage I to stage IV melanosomes.

Post-translational modifications:
The delta, brain-specific form differs from the zeta form in being phosphorylated (By similarity). Phosphorylation on Ser-184 by MAPK8; promotes dissociation of BAX and translocation of BAX to mitochondria. Phosphorylation on Ser-58 by PKA; disrupts homodimerization and heterodimerization with YHAE and TP53. This phosphorylation appears to be activated by sphingosine. Phosphorylation on Thr-232; inhibits binding of RAF1.

Similarity:
Belongs to the 14-3-3 family.

SWISS:
P63104

Gene ID:
7534

Database links:

Entrez Gene: 7529 Human

Entrez Gene: 7534 Human

Entrez Gene: 22631 Mouse

Entrez Gene: 5881 Mouse

Entrez Gene: 25578 Rat

Entrez Gene: 56011 Rat

Omim: 601288 Human

Omim: 601289 Human

SwissProt: P29358 Cow

SwissProt: P31946 Human

SwissProt: P63104 Human

SwissProt: P35215 Mouse

SwissProt: Q9CQV8 Mouse

SwissProt: P35213 Rat

SwissProt: P63102 Rat

SwissProt: P29361 Sheep

Unigene: 49487 Human

Unigene: 643544 Human

Unigene: 1292 Rat

Unigene: 8653 Rat

Picture

Sample:
Cerebrum (Rat) Lysate at 40 ug
Primary: Anti-14-3-3 zeta+delta (SL6790R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 27 kD
Observed band size: 27 kD

Blank control (blue line): HL60 (fixed with 70% ethanol (overninght at 4℃) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature).
Primary Antibody (green line): Rabbit Anti-14-3-3 zeta+delta antibody (SL6790R),Dilution: 0.2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE<, Dilution: 1μg /test.

Blank control (black line): Raji (black) (The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with PBST for 30 min on room temperature)
Primary Antibody (rad line): Rabbit Anti-14-3-3 zeta+delta antibody (SL6790R) ; Dilution: 1μg /10^6 cells;
Isotype Control Antibody (grenn line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE;Dilution: 1μg /test.

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