Rabbit Anti-Anillin antibody

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Actin binding protein scraps homolog Drosophila; Actin binding protein anillin; Anillin actin binding protein; ANLN; DKFZp779A055; Scra; Scraps; Scraps homolog.

Cat:

SL7738R

Species Reactivity：

Human,Mouse,(predicted: Rat,Dog,Cow,Horse,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human Anillin:501-600/1124

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=1ug/testICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Paraformaldehyde-fixed, paraffin embedded (Mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Anillin) Polyclonal Antibody, Unconjugated (SL7738R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Anillin) Polyclonal Antibody, Unconjugated (SL7738R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Tissue/cell: human placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-Anillin Polyclonal Antibody, Unconjugated(SL7738R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingBlank control:A431. Primary Antibody (green line): Rabbit Anti-Anillin antibody (SL7738R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647Dilution: 1μg /test. ProtocolThe cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Anillin, also known as scraps homolog, is an evolutionarily conserved Actin- binding protein required for cytokinesis that was first identified in Drosophila melanogaster. Anillin is a ubiquitously expressed protein with highest expression levels in the central nervous system. It is predominantly found in the nucleus and it localizes to the cleavage furrow during cytokinesis, forming a ring with the help of Rac GTPase. During cytokinesis, Anillin interacts with CD2AP and functions to concentrate Rho A and maintain the localization of active Myosin. In Anillin knockout cells the cleavage furrow fails to complete ingression. Anillin expression levels fluctuate with the cell cycle, peaking in mitosis. Before the cell exits into G1, Anillin associates with E-cadherin and is ubiquitinated by the anaphase-promoting complex/cyclosome (APC/C). APC/C recognizes the D-box domain at the N-terminal region of Anillin. Anillin is commonly overexpressed in tumors and may serve as a potential biomarker.Anillin is required for cytokinesis. It is essential for the structural integrity of the cleavage furrow and for completion of cleavage furrow ingression.

Function:
Required for cytokinesis. Essential for the structural integrity of the cleavage furrow and for completion of cleavage furrow ingression.

Subunit:
Interacts with F-actin. Interacts with CD2AP. May interact with RHOA. Interacts with FZR1/CDH1 during mitotic exit.

Subcellular Location:
Nucleus. Cytoplasm, cytoskeleton. Cytoplasm, cell cortex. Note=Mainly found in the nucleus during interphase. Colocalizes with cortical F-actin upon nuclear envelope breakdown in mitosis and subsequently concentrates in the area of the prospective contractile ring in anaphase. This pattern persists until telophase, when the protein becomes concentrated in the midbody.

Tissue Specificity:
Ubiquitously expressed. Present at highest levels in the brain, at high levels in the placenta and testis, at intermediate levels in the intestine, ovary, skeletal muscle and thymus and at lower levels in heart, kidney, liver, lung, pancreas, prostate and spleen. Overexpressed in many tumor types including breast, colorectal, endometrial, hepatic, kidney, lung, ovarian and pancreatic tumors.

Post-translational modifications:
Phosphorylated during mitosis.
Ubiquitinated, and this requires FZR1/CDH1.

Similarity:
Contains 1 PH domain.

SWISS:
Q9NQW6

Gene ID:
54443

Picture

Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Anillin) Polyclonal Antibody, Unconjugated (SL7738R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Tissue/cell: human placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-Anillin Polyclonal Antibody, Unconjugated(SL7738R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Blank control:A431.
Primary Antibody (green line): Rabbit Anti-Anillin antibody (SL7738R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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