Rabbit Anti-KATNA1 antibody

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Katanin p60 A1; Katanin p60 ATPase containing subunit A1; Katanin p60 ATPase-containing subunit A1; Katanin p60 subunit A1; KATNA1; KTNA1_HUMAN; p60 katanin.

Cat:

SL9308R

Species Reactivity：

Human,Mouse,(predicted: Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human KATNA1/Katanin p60 A1:221-320/491

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:50-200（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Fetal brain (Mouse) Lysate at 40 ugPrimary: Anti-KATNA1 (SL9308R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size:56 kDObserved band size: 56 kDSample: Hela(Human) Cell Lysate at 30 ugHepG2(Human) Cell Lysate at 30 ugCerebral cortex (Mouse) Lysate at 40 ugPrimary: Anti-KATNA1 (SL9308R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size:56 kDObserved band size: 56 kD Paraformaldehyde-fixed, paraffin embedded (Human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KATNA1) Polyclonal Antibody, Unconjugated (SL9308R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KATNA1) Polyclonal Antibody, Unconjugated (SL9308R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Microtubules are polymers of alpha and beta subunits that form the mitotic spindle and assist in the organization of membranous organelles during interphase. Katanin p60 A1, also known as KATNA1, is a 491 amino acid protein that belongs to the AAA ATPase family and is involved in microtubule regulation. Localized to the cytoplasm and to the centrosome, Katanin p60 A1 functions to sever and disassemble microtubules in an ATP-dependent manner, thus promoting the rapid reorganization of cellular microtubule arrays and playing an important role in microtubule release from the centrosome after nucleation. Katanin p60 A1, which exists as two alternatively spliced isoforms, can homooligomerize into hexameric rings whose activity is stimulated by the presence of microtubules.

Function:
Catalytic subunit of a complex which severs microtubules in an ATP-dependent manner. Microtubule severing may promote rapid reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. Microtubule release from the mitotic spindle poles may allow depolymerization of the microtubule end proximal to the spindle pole, leading to poleward microtubule flux and poleward motion of chromosome. Microtubule release within the cell body of neurons may be required for their transport into neuronal processes by microtubule-dependent motor proteins. This transport is required for axonal growth (By similarity).

Subunit:
Can homooligomerize into hexameric rings, which may be promoted by interaction with microtubules. Interacts with KATNB1, which may serve as a targeting subunit. Interacts with dynein and NDEL1. Associates with the E3 ligase complex containing DYRK2, EDD/UBR5, DDB1 and VPRBP proteins (EDVP complex).

Subcellular Location:
Cytoplasm. Cytoplasm, cytoskeleton, centrosome. Cytoplasm, cytoskeleton, spindle pole. Cytoplasm, cytoskeleton. Note=Predominantly cytoplasmic. Also localized to the interphase centrosome and the mitotic spindle poles. Enhanced recruitment to the mitotic spindle poles requires microtubules and interaction with KATNB1.

Post-translational modifications:
Ubiquitinated by the EDVP E3 ligase complex and subsequently targeted to proteasomal degradation.

Similarity:
Belongs to the AAA ATPase family. Katanin p60 subunit A1 subfamily.

SWISS:
O75449

Gene ID:
11104

Database links:

Entrez Gene: 11104 Human

Entrez Gene: 23924 Mouse

Omim: 606696 Human

SwissProt: O75449 Human

SwissProt: Q9WV86 Mouse

Unigene: 450175 Human

Unigene: 28127 Mouse

Unigene: 421297 Mouse

Picture

Sample:
Hela(Human) Cell Lysate at 30 ug
HepG2(Human) Cell Lysate at 30 ug
Cerebral cortex (Mouse) Lysate at 40 ug
Primary: Anti-KATNA1 (SL9308R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size:56 kD
Observed band size: 56 kD

Paraformaldehyde-fixed, paraffin embedded (Human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KATNA1) Polyclonal Antibody, Unconjugated (SL9308R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KATNA1) Polyclonal Antibody, Unconjugated (SL9308R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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