Rabbit Anti-Neutrophil Elastase antibody

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Bone Marrow Serine Protease; ELA 2; ELA2; ELANE; Elastase 2; Elastase-2; Elastase 2 neutrophil; Elastase neutrophil expressed; Elastase-2; ELNE_HUMAN; GE antibody Granulocyte derived elastase; HLE; HNE; Human leukocyte elastase; Leukocyte elastase; Leukoc

Cat:

SL6982R

Species Reactivity：

Human,Mouse,Rat,(predicted: Dog,)

Immunogen：

KLH conjugated synthetic peptide derived from human Neutrophil Elastase/ELANE:101-200/267

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000Flow-Cyt=1μg/Testnot yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Spinal cord (Mouse) Lysate at 40 ugPrimary: Anti-Neutrophil Elastase (SL6982R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 26 kDObserved band size: 48 kDSample: Lane 1: Bone (Mouse) Tissue Lysate at 40 ugLane 2: Bone (Rat) Tissue Lysate at 40 ugPrimary: Anti-Neutrophil Elastase (SL6982R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 26 kDObserved band size: 40 kDSample: HL60(Human) Cell Lysate at 30 ugPrimary: Anti-Neutrophil Elastase (SL6982R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 26 kDObserved band size: 41 kDSample: Cerebrum (Mouse) Lysate at 40 ugPrimary: Anti-Neutrophil Elastase (SL6982R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 26 kDObserved band size: 31/46 kDBlank control: THP-1. Primary Antibody (green line): Rabbit Anti-Neutrophil Elastase antibody (SL6982R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-FITCDilution: 0.5μg /test. ProtocolThe cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.Blank control: A549(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice..Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μl in 100 μL1X PBS containing 0.5% BSA(green).

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins. The product of this gene hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix following the protein's release from activated neutrophils. The enzyme may play a role in degenerative and inflammatory diseases by its proteolysis of collagen-IV and elastin of the extracellular matrix. This protein degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is clustered with other serine protease gene family members, azurocidin 1 and proteinase 3 genes, at chromosome 19pter. All 3 genes are expressed coordinately and their protein products are packaged together into azurophil granules during neutrophil differentiation. [provided by RefSeq, May 2009].

Function:
Modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis.

Subunit:
Interacts with NOTCH2NL.

Tissue Specificity:
Bone marrow cells.

DISEASE:
Defects in ELANE are a cause of cyclic haematopoiesis (CH) [MIM:162800]; also known as cyclic neutropenia. CH is an autosomal dominant disease in which blood-cell production from the bone marrow oscillates with 21-day periodicity. Circulating neutrophils vary between almost normal numbers and zero. During intervals of neutropenia, affected individuals are at risk for opportunistic infection. Monocytes, platelets, lymphocytes and reticulocytes also cycle with the same frequency.
Defects in ELANE are the cause of neutropenia severe congenital autosomal dominant type 1 (SCN1) [MIM:202700]. SCN1 is a disorder of hematopoiesis characterized by a maturation arrest of granulopoiesis at the level of promyelocytes with peripheral blood absolute neutrophil counts below 0.5 x 10(9)/l and early onset of severe bacterial infections.

Similarity:
Belongs to the peptidase S1 family. Elastase subfamily. Contains 1 peptidase S1 domain.

SWISS:
P08246

Gene ID:
1991

Database links:

Entrez Gene: 1991 Human

Entrez Gene: 50701 Mouse

Omim: 130130 Human

SwissProt: P08246 Human

SwissProt: Q3UP87 Mouse

Unigene: 99863 Human

Unigene: 262194 Mouse

Picture

Sample:
Lane 1: Bone (Mouse) Tissue Lysate at 40 ug
Lane 2: Bone (Rat) Tissue Lysate at 40 ug
Primary:
Anti-Neutrophil Elastase (SL6982R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 26 kD
Observed band size: 40 kD

Sample:
HL60(Human) Cell Lysate at 30 ug
Primary: Anti-Neutrophil Elastase (SL6982R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 26 kD
Observed band size: 41 kD

Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti-Neutrophil Elastase (SL6982R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 26 kD
Observed band size: 31/46 kD

Blank control: THP-1.
Primary Antibody (green line): Rabbit Anti-Neutrophil Elastase antibody (SL6982R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Blank control: A549(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice..
Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μl in 100 μL1X PBS containing 0.5% BSA(green).

Product Feedback Wall

Specific References (14) | SL6982R has been referenced in 14 publications.

[IF=1.704] Störmann P et al. Contributing factors in the development of acute lung injury in a murine double hit model. Eur J Trauma Emerg Surg. 2019 Apr 1. IHSLCP ; Mouse.

PubMed:3093792

[IF=20.479] Andrew R. Sas. et al. A new neutrophil subset promotes CNS neuron survival and axon regeneration. Nat Immunol. 2020 Oct;21(12):1496-1505 FC ; Mouse.

PubMed:33106668

[IF=13.116] Vaibhav K et al. Neutrophil extracellular traps exacerbate neurological deficits after traumatic brain injury. Sci Adv.2020 May 29;6(22):eaax8847. FCM ; Mouse.

PubMed:3252796

[IF=4.12] Arecco et al. Elastase levels and activity are increased in dystrophic muscle and impair myoblast cell survival, proliferation and differentiation. (2016) Sci.Rep. 6:24708 IF,WB ; Mouse.

PubMed:27241590

[IF=3.46] Peng et al. Adiponectin and adiponectin receptor 1 overexpression enhance inflammatory bowel disease. (2018) J.Biomed.Sci. 25:24 IHC ; Mouse.

PubMed:29540173

[IF=5.23] Arecco, N., et al. "Elastase levels and activity are increased in dystrophic muscle and impair myoblast cell survival, proliferation and differentiation."Scientific Reports 6 (2016): 24708. IHSLCP, WB ; Mouse.

PubMed:27241590

[IF=2.35] Li, Guofu, et al. "The neutrophil elastase inhibitor, sivelestat, attenuates sepsis-related kidney injury in rats." International Journal of Molecular Medicine 38.3 (2016): 767-775. WB ; Rat.

PubMed:27430552

[IF=2.26] Maekawa, Toshihiro, et al. "Prophylactic Effect of Lactobacillus pentosus strain S-PT84 on Candida Infection and Gastric Inflammation in a Murine Gastrointestinal Candidiasis Model [Errata]." Medical mycology journal 57.4 (2016): E81-E92. IHSLCP ; Mouse.

PubMed:27904074

[IF=7.793] Gao J et al. Metformin protects against PM2. 5-induced lung injury and cardiac dysfunction independent of AMP-activated protein kinase α2. Redox Biol. 2019 Oct 19;28:101345. IHSLCP ; Mouse.

PubMed:31669973

[IF=7.793] Wang H et al. The effect of exposure time and concentration of airborne PM2. 5 on lung injury in mice: A transcriptome analysis. Redox Biol. 2019 Jul 2;26:101264. IHSLCP ; Mouse.

PubMed:31279222

[IF=3.457] Ye, Y. et al. CXCR1/CXCR2 antagonist G31P inhibits nephritis in a mouse model of uric acid nephropathy.(2018) BIOMED PHARMACOTHER IHC ; mouse.

PubMed:10.1016/j.biopha.2018.07.077

[IF=6.02] Wang H et al. AMPKα2 deficiency exacerbates long-term PM2.5 exposure-induced lung injury and cardiac dysfunction.Free Radic Biol Med. 2018 Jun;121:202-214. WB ; Mouse,Human&Rat.

PubMed:29753072

[IF=4.22] Krishnan, Subramanian, et al. "Serotype O18 avian pathogenic and neonatal meningitis Escherichia coli strains employ similar pathogenic strategies for the onset of meningitis." Virulence 6.8 (2015): 777-786. IHSLCP ; Mouse.

PubMed:21287066

[IF=1.53] Jeffery, Unity, et al. "Dogs Cast NETs too: Canine neutrophil extracellular traps in health and immune-mediated hemolytic anemia." Veterinary Immunology and Immunopathology (2015). Dog.

PubMed:26574161