Rabbit Anti-CD3E antibody | Sunlong Medical

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CD3 epsilon; CD3e; CD3e antigen epsilon polypeptide (TiT3 complex); CD3E antigen epsilon polypeptide; CD3E antigen, epsilon subunit; CD3e molecule epsilon; CD3e molecule, epsilon (CD3 TCR complex); CD3e molecule, epsilon (CD3-TCR complex); CD3E_HU

Cat:

SL4815R

Species Reactivity：

Mouse,(predicted: Rat,)

Immunogen：

KLH conjugated synthetic peptide derived from mouse CD3 epsilon:23-120/189<Extracellular>

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000Flow-Cyt=2ug/Testnot yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Spleen (Mouse) Lysate at 40 ugPrimary: Anti-CD3E (SL4815R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 20 kDObserved band size: 20 kDBlank control:Mouse bone marrow. Primary Antibody (green line): Rabbit Anti-CD3E antibody (SL4815R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647Dilution: 1μg /test. ProtocolThe cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.Blank control: mouse blood Isotype Control Antibody: Rabbit IgG -PE-CY5; Primary Antibody Dilution: 1 μl in 100 μL1X PBS containing 0.5% BSA.Blank control (blue line): Mouse spleen cells(fixed with 70% ice-cold methanol overnight at 4℃). Primary Antibody (green line): Rabbit Anti-CD3E/PE-CY7 Conjugated antibody (SL4815R-PE-CY7),Dilution: 1μg /10^6 cells. Isotype Control Antibody (orange line): Rabbit IgG-PE-CY7 .

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008].

Function:
The CD3 complex mediates signal transduction.

Subunit:
The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta.

Subcellular Location:
Membrane; Single-pass type I membrane protein.

Similarity:
Contains 1 Ig-like (immunoglobulin-like) domain.
Contains 1 ITAM domain.

SWISS:
P22646

Gene ID:
12501

Database links:

Entrez Gene: 916 Human

Entrez Gene: 12501 Mouse

Omim: 186830 Human

SwissProt: P07766 Human

SwissProt: P22646 Mouse

Unigene: 3003 Human

Unigene: 210361 Mouse

Picture

Blank control:Mouse bone marrow.
Primary Antibody (green line): Rabbit Anti-CD3E antibody (SL4815R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Blank control: mouse blood
Isotype Control Antibody: Rabbit IgG -PE-CY5; Primary Antibody Dilution: 1 μl in 100 μL1X PBS containing 0.5% BSA.

Blank control (blue line): Mouse spleen cells(fixed with 70% ice-cold methanol overnight at 4℃).
Primary Antibody (green line): Rabbit Anti-CD3E/PE-CY7 Conjugated antibody (SL4815R-PE-CY7),Dilution: 1μg /10^6 cells.
Isotype Control Antibody (orange line): Rabbit IgG-PE-CY7 .

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