Sample:
Cerebrum (Rat) Lysate at 40 ug
Primary: Anti-GABR B3 (SL8544R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kD
Observed band size: 52 kD
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Hippocampus (Mouse) Lysate at 40 ug
Primary: Anti-GABR B3 (SL8544R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kD
Observed band size: 52 kD
Sample: Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti-GABR B3 (SL8544R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kD
Observed band size: 48 kD
Blank control(blue):RSC96 (fixed with 2% paraformaldehyde (10 min)). Primary Antibody:Rabbit Anti- GABR B3 antibody(SL8544R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
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