Rabbit Anti-Wnt11 antibody | Sunlong Medical

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HWNT11; Protein Wnt-11; Wingless-related MMTV integration site 11; Wingless-type MMTV integration site family, member 1; Wingless-type MMTV integration site family, member 11; Wingless-type MMTV integration site family, member 11B; wnt-11; wnt11b.WNT11_HU

Cat:

SL8568R

Species Reactivity：

Mouse,(predicted: Human,Rat,Dog,Pig,Cow,Horse,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human Wnt11:151-250/354

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Embryo (Mouse) Lysate at 40 ug293T(Human) Cell Lysate at 30 ugPrimary: Anti- Wnt11 (SL8568R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 36 kDObserved band size: 35 kDSample: Liver (Mouse) Lysate at 40 ugPrimary: Anti- Wnt11 (SL8568R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 36 kDObserved band size: 63 kDSample: Heart (Mouse) Lysate at 40 ugPrimary: Anti- Wnt11 (SL8568R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 36 kDObserved band size: 34 kDParaformaldehyde-fixed, paraffin embedded (mouse liver tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Wnt11) Polyclonal Antibody, Unconjugated (SL8568R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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The Wnt genes belong to a family of protooncogenes with at least 13 known members that are expressed in species ranging from Drosophila to man. The name Wnt denotes the relationship of this family to the Drosophila segment polarity gene ”wingless” and to its vertebrate ortholog, Int-1, a mouse proto-oncogene. Transcription of Wnt family genes appears to be developmentally regulated in a precise temporal and spatial manner. The Wnt genes encode cysteine-rich putative glycoproteins which have features typical of secreted growth factors. Wnt-11 is expressed in the tips of ureteric buds and in the perichondrium, a stem cell-like layer that surrounds the future bones and directs their growth and regeneration. Wnt-11 activity is required for cells to undergo correct convergent extension movements during gastrulation. Human Wnt-11 is also expressed in the lung mesenchyme, the urorectal septum, the urogenital folds, the labioscrotal swellings, and the cortex of the adrenal gland. Unlike other Wnt family members, Wnt-11 is not expressed in the neuroepithelium of the central nervous system. Wnt-11, along with Wnt-8c, is ex-pressed in the posterior region of the chick embryo in the caudal paraxial mesoderm that underlies the prospective caudal neural plate. The gene which encodes Wnt-11 maps to human chromosome 11q13.5.

Function:
Wnt11 is a non-canonical Wnt which is expressed in androgen-independent prostate cancer cell lines and prostate tumours. It is involved in development, including cardiac and gut development.

Subcellular Location:
Secreted; extracellular space; extracellular matrix.

Tissue Specificity:
Expressed in fetal lung, kidney, adult heart, liver, skeletal muscle, and pancreas.

Post-translational modifications:
Palmitoylation at Ser-215 is required for efficient binding to frizzled receptors. It is also required for subsequent palmitoylation at Cys-80. Palmitoylation is necessary for proper trafficking to cell surface (By similarity).

Similarity:
Belongs to the Wnt family.

SWISS:
O96014

Gene ID:
7481

Database links:

Entrez Gene: 7481 Human

Entrez Gene: 22411 Mouse

Entrez Gene: 140584 Rat

Omim: 603699 Human

SwissProt: O96014 Human

SwissProt: P48615 Mouse

Picture

Sample:
Liver (Mouse) Lysate at 40 ug
Primary: Anti- Wnt11 (SL8568R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kD
Observed band size: 63 kD

Sample:
Heart (Mouse) Lysate at 40 ug
Primary: Anti- Wnt11 (SL8568R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kD
Observed band size: 34 kD

Paraformaldehyde-fixed, paraffin embedded (mouse liver tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Wnt11) Polyclonal Antibody, Unconjugated (SL8568R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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