C9orf86 (Partner of ARF;PARF) binds p19(ARF), but not the p16(INK4A) isoform of CDKN2A. Knockdown of C9orf86 by RNA interference reduced the growth inhibitory activity of p19(ARF) and enhanced the rate of cell proliferation.
Function:
May enhance cellular proliferation. May reduce growth inhibitory activity of CDKN2A.
Subcellular Location:
Cytoplasmic and Nuclear. Note=Predominantly cytoplasmic. Proposed to shuttle between cytoplasm and nucleus.
Post-translational modifications:
Isoform 1 is O-glycosylated, while other isoforms are not.
Similarity:
Belongs to the small GTPase superfamily. Rab family.
SWISS:
Q3YEC7
Gene ID:
55684
Database links:
Entrez Gene: 55684 Human
Entrez Gene: 227624 Mouse
Entrez Gene: 227624 Rat
Omim: 610615 Human
SwissProt: Q3YEC7 Human
SwissProt: Q5U3K5 Mouse
Unigene: 370555 Human
Unigene: 29600 Mouse
Unigene: 443064 Mouse
Unigene: 17709 Rat
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Sample:
Stomach (Mouse) Lysate at 40 ug
Primary: Anti- C9orf86 (SL9545R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 80 kD
Observed band size: 80 kD
Paraformaldehyde-fixed, paraffin embedded (mouse stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (C9orf86) Polyclonal Antibody, Unconjugated (SL9545R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (C9orf86) Polyclonal Antibody, Unconjugated (SL9545R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (C9orf86) Polyclonal Antibody, Unconjugated (SL9545R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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