Rabbit Anti-PCDH20 antibody

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PCD20_HUMAN; PCDH 13; PCDH 20; PCDH13; PCDH20; Protocadherin 13; Protocadherin 20; Protocadherin-13; Protocadherin-20; Protocadherin13; Protocadherin20.

Cat:

SL11113R

Species Reactivity：

Human,Mouse,Rat,(predicted: Horse,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human PCDH20:611-710/924<Extracellular>

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500Flow-Cyt=1μg/Test（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: MCF-7(Human) Cell Lysate at 30 ugPrimary: Anti-PCDH20 (SL11113R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 98 kDObserved band size: 100 kDSample: MDA-MB-231 Cell (Human) Lysate at 40 ugPrimary: Anti-PCDH20 (SL11113R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 98 kDObserved band size: 98 kDSample: Lane 1: Human SH-SY5Y cell lysates Lane 2: Human U251 cell lysates Lane 3: Human U87MG cell lysates Primary: Anti-PCDH20 (SL11113R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 98 kDaObserved band size: 102 kDaSample: Hippocampus (Mouse) Lysate at 40 ugOlfactory bulb (Mouse) Lysate at 40 ugPrimary: Anti-PCDH20 (SL11113R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 98 kDObserved band size: 100 kDParaformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SV2B) Polyclonal Antibody, Unconjugated (SL11113R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Blank control(blue): RSC96 (fixed with 2% paraformaldehyde (10 min)). Primary Antibody:Rabbit Anti- PCDH20 antibody(SL11113R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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As a subfamily of the cadherin superfamily, protocadherins are cadherin-like cell adhesion proteins that contain up to seven extracellular domains and are predominantly expressed in the nervous system. Importantly, the adhesion mechanism of protocadherins is distinct from classic cadherins. Through inactivation or overexpression, several protocadherins have been implicated in a variety of cancers. Protocadherin-20 (PCDH20), also known as protocadherin-13, is a 924 amino acid protein containing 6 cadherin domains and potentially functioning as a calcium-dependent cell-adhesion protein. In non-small cell lung cancer cell lines, a homozygous loss of PCDH20 was identified through either deletion of one allele and methylation of the other or methylation of both alleles. Hypermethylation of PCDH20 is associated with worse prognosis and clinical outcome, suggesting that PCDH20 may function as a tumor suppressor.

Function:
Potential calcium-dependent cell-adhesion protein.

Subcellular Location:
Cell membrane.

Similarity:
Contains 6 cadherin domains.

SWISS:
Q8N6Y1

Gene ID:
64881

Database links:

Entrez Gene: 64881 Human

Omim: 614449 Human

SwissProt: Q8N6Y1 Human

Unigene: 391781 Human

Picture

Sample: MDA-MB-231 Cell (Human) Lysate at 40 ug
Primary: Anti-PCDH20 (SL11113R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 98 kD
Observed band size: 98 kD

Sample:
Lane 1: Human SH-SY5Y cell lysates
Lane 2: Human U251 cell lysates
Lane 3: Human U87MG cell lysates
Primary: Anti-PCDH20 (SL11113R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 98 kDa
Observed band size: 102 kDa

Sample:
Hippocampus (Mouse) Lysate at 40 ug
Olfactory bulb (Mouse) Lysate at 40 ug
Primary: Anti-PCDH20 (SL11113R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 98 kD
Observed band size: 100 kD

Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SV2B) Polyclonal Antibody, Unconjugated (SL11113R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Blank control(blue): RSC96 (fixed with 2% paraformaldehyde (10 min)).
Primary Antibody:Rabbit Anti- PCDH20 antibody(SL11113R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

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