Rabbit Anti-PVRL1 antibody | Sunlong Medical

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CD111; CD111 antigen; CLPED1; ectodermal dysplasia 4 (Margarita Island type); ED4; Herpes virus entry mediator C; Herpesvirus entry mediator C; Herpesvirus Ig like receptor; Herpesvirus Ig-like receptor; HIgR; HveC; Nectin 1; Nectin-1; Nectin1; OFC7; ORO

Cat:

SL11126R

Species Reactivity：

Human,Mouse,Rat,(predicted: Dog,Cow,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human PVRL1/CD111/Nectin1:31-130/517<Extracellular>

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Lane 1: Human U87MG cell lysates Lane 2: Human SH-SY5Y cell lysates Primary: Anti-PVRL1 (SL11126R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 54 kDaObserved band size: 54 kDaSample: Lane 1: Mouse Urinary bladder tissue lysates Lane 2: Rat Urinary bladder tissue lysates Primary: Anti-PVRL1 (SL11126R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 54 kDaObserved band size: 57 kDaSample: Testis(Mouse) Lysate at 40 ugPrimary: Anti-PVRL1 (SL11126R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 54 kDObserved band size: 54 kDSample: MCF-7 Cell (Human) Lysate at 30 ugPrimary: Anti-PVRL1 (SL11126R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 54 kDObserved band size: 57 kD Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PVRL1) Polyclonal Antibody, Unconjugated (SL11126R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PVRL1) Polyclonal Antibody, Unconjugated (SL11126R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Nectin is a Ca2+-independent homophilic cell adhesion molecule that belongs to the immunoglobulin superfamily. Human Nectin is identical to the poliovirus receptor-related protein (PRR) and is identified to be the alphaherpesvirus entry mediator. Nectin constitutes a family consisting of at least nectin 1, 2 and 3. Nectin 2 and 3 are ubiquitously expressed, whereas nectin 1 is abundantly expressed in the brain. Nectin 1 exists as nectin 1å and 1∫/HIgR, produced by alternative splicing. The cytoplasmic regions of Nectin 1å, but not Nectin 1∫/HIgR, have a SLCterminal conserved motif (E/A-X-Y-V). This motif interacts with the PDZ domain of the F-Actin-binding protein, afadin, through which it is linked to the Actin cytoskeleton. Nectin 1, also designated HveC/ PRR1, allows the entry of herpes simplex virus type 1 (HSSLV1) and HSSLV2 into mammalian cells. The interaction of virus envelope glycoprotein D (gD) with nectin 1 is an essential step in the process leading to membrane fusion; the gD binding site is located at the first Ig-like domain of Nectin 1. Both the transinteraction of nectin and the interaction of nectin with afadin are necessary for their co-localization with E-cadherin and catenins at adherens junctions.

Function:
Promotes cell-cell contacts by forming homophilic or heterophilic trans-dimers. Heterophilic interactions have been detected between PVRL1/nectin-1 and PVRL3/nectin-3 and between PVRL1/nectin-1 and PVRL4/nectin-4.

Subunit:
Can form trans-heterodimers with PVRL3/nectin-3 and with PVRL4/nectin-4. Interacts (via SLCterminus) with afadin (via PDZ domain); this interaction recruits PVRL1 to cadherin-based adherens junctions. Interacts with integrin alphaV/beta3. Interacts with herpes simplex virus 1 (HHSLV1), herpes simplex virus 2 (HHSLV2), and pseudorabies virus (PRV) envelope glycoprotein D; functions as an entry receptor for these viruses.

Subcellular Location:
Isoform Alpha: Cell membrane; Single-pass type I membrane protein. Isoform Delta: Cell membrane; Single-pass type I membrane protein. Isoform Gamma: Secreted.

DISEASE:
Defects in PVRL1 are the cause of ectodermal dysplasia Margarita Island type (EDMI) [MIM:225060]; also known as Zlotogora-Ogur syndrome, cleft lip/palate-ectodermal dysplasia syndrome (CLPED1) or ectodermal dysplasia 4. Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. EDMI is an autosomal recessive syndrome characterized by the association of cleft lip/palate, ectodermal dysplasia (sparse short and dry scalp hair, sparse eyebrows and eyelashes), and partial syndactyly of the fingers and/or toes. Two thirds of the patients do not manifest oral cleft but present with abnormal teeth and nails.

Similarity:
Belongs to the nectin family.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like SLVtype (immunoglobulin-like) domain.

SWISS:
Q15223

Gene ID:
5818

Database links:

Entrez Gene: 5818 Human

Entrez Gene: 58235 Mouse

Entrez Gene: 397247 Pig

Entrez Gene: 192183 Rat

Omim: 600644 Human

SwissProt: Q15223 Human

SwissProt: Q9JKF6 Mouse

SwissProt: Q9GL76 Pig

Unigene: 334846 Human

Unigene: 335096 Mouse

Picture

Sample:
Lane 1: Mouse Urinary bladder tissue lysates
Lane 2: Rat Urinary bladder tissue lysates
Primary: Anti-PVRL1 (SL11126R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kDa
Observed band size: 57 kDa

Sample:
Testis(Mouse) Lysate at 40 ug
Primary: Anti-PVRL1 (SL11126R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kD
Observed band size: 54 kD

Sample:
MCF-7 Cell (Human) Lysate at 30 ug
Primary: Anti-PVRL1 (SL11126R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kD
Observed band size: 57 kD

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PVRL1) Polyclonal Antibody, Unconjugated (SL11126R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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