Glutamate receptors mediate most excitatory neurotransmission in the brain and play an important role in neural plasticity, neural development and neurodegeneration. Ionotropic glutamate receptors are categorized into NMDA receptors and kainate/AMPA receptors, both of which contain glutamate-gated, cation-specific ion channels. Kainate/AMPA receptors are co-localized with NMDA receptors in many synapses and consist of seven structurally related subunits designated GluR-1 to -7. The kainate/AMPA receptors are primarily responsible for the fast excitatory neuro-transmission by glutamate whereas the NMDA receptors are functionally characterized by a slow kinetic and a high permeability for Ca2+ ions. The NMDA receptors consist of five subunits: epsilion 1, 2, 3, 4 and one zeta subunit. The zeta subunit is expressed throughout the brainstem whereas the four epsilon subunits display limited distribution.
Function:
Receptor for glutamate. The activity of this receptor is mediated by a G-protein that inhibits adenylate cyclase activity.
Subunit:
nteracts with GRASP
Subcellular Location:
Cell membrane; Multi-pass membrane protein.
Similarity:
Belongs to the G-protein coupled receptor 3 family.
SWISS:
Q14832
Gene ID:
2913
Database links:
Entrez Gene: 2913 Human
Entrez Gene: 108069 Mouse
Entrez Gene: 24416 Rat
Omim: 601115 Human
SwissProt: Q14832 Human
SwissProt: Q9QYS2 Mouse
SwissProt: P31422 Rat
Unigene: 590575 Human
Unigene: 318966 Mouse
Unigene: 41715 Rat
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Sample:
Hippocampus (Mouse) Lysate at 40 ug
Primary: Anti-MGLUR3 (SL12012R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 96 kD
Observed band size: 116 kD
Sample:
Cerebral cortex (Mouse) Lysate at 40 ug
Primary: Anti-MGLUR3 (SL12012R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 96 kD
Observed band size: 116 kD
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti-MGLUR3 (SL12012R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 96 kD
Observed band size: 116 kD
Blank control: Mouse Liver Cells(fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice).
Primary Antibody: Rabbit Anti- MGLUR3/PE Conjugated antibody (SL12012R-PE), Dilution: 5μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG/PE(orange) ,used under the same conditions.
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