Rabbit Anti-ST18 antibody | Sunlong Medical

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KIAA0535; St18; ST18_HUMAN; suppression of tumorigenicity 18 (breast carcinoma) (zinc finger protein); Suppression of tumorigenicity 18 protein; Suppression of tumorigenicity protein 18; Zinc finger protein 387; Znf387.

Cat:

SL12188R

Species Reactivity：

Human,Mouse,Rat,(predicted: Dog,Horse,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human ST18/Znf387:451-550/1047

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=3ug/TestICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Cerebrum (Mouse) Lysate at 40 ugCerebrum (Rat) Lysate at 40 ugPrimary: Anti- ST18 (SL12188R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 115 kDObserved band size: 110 kDSample: U87MG(Human) Cell Lysate at 30 ugPrimary: Anti- ST18 (SL12188R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 115 kDObserved band size: 115 kDTissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-ST18 Polyclonal Antibody, Unconjugated(SL12188R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingBlank control: Mouse kidney. Primary Antibody (green line): Rabbit Anti-ST18 antibody (SL12188R) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PEDilution: 1μg /test. ProtocolThe cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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ST18 is a 1,047 amino acid nuclear protein that is expressed at low levels in heart, liver, kidney, skeletal muscle, pancreas, testis, ovary and prostate. Containing six C2HSLCtype zinc fingers and an SMC domain, ST18 is a repressor protein that binds to DNA sequences containing a specific bipartite element. ST18 is considered a transcription factor that represses basal transcription activity from target promoters and Inhibits colony formation in cultured breast cancer cells. ST18 is encoded by a gene located on human chromosome 8, which consists of nearly 146 million base pairs, encodes over 800 genes and is associated with a variety of diseases and malignancies. Schizophrenia, bipolar disorder, Trisomy 8, Pfeiffer syndrome, congenital hypothyroidism, Waardenburg syndrome and some leukemias and lymphomas are thought to occur as a result of defects in specific genes that map to chromosome 8.

Function:
Repressor that binds to DNA sequences containing a bipartite element consisting of a direct repeat of the sequence 5'-AAAGTTT-3' separated by 2-9 nucleotides. Represses basal transcription activity from target promoters (By similarity). Inhibits colony formation in cultured breast cancer cells.

Subcellular Location:
Nucleus.

Tissue Specificity:
Detected at low levels in heart, liver, kidney, skeletal muscle, pancreas, testis, ovary and prostate. Detected at even lower levels in mammary epithelial cells and breast cancer cells.

Similarity:
Contains 6 C2HSLCtype zinc fingers.

SWISS:
O60284

Gene ID:
9705

Database links:

Entrez Gene: 9705 Human

Entrez Gene: 48690 Mouse

Entrez Gene: 266136 Rat

SwissProt: O60284 Human

SwissProt: Q80TY4 Mouse

SwissProt: Q9QX27 Rat

Unigene: 655499 Human

Unigene: 234612 Mouse

Unigene: 91311 Rat

Picture

Sample:
U87MG(Human) Cell Lysate at 30 ug
Primary: Anti- ST18 (SL12188R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 115 kD
Observed band size: 115 kD

Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-ST18 Polyclonal Antibody, Unconjugated(SL12188R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Blank control: Mouse kidney.
Primary Antibody (green line): Rabbit Anti-ST18 antibody (SL12188R)
Dilution: 3μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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