Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin. [provided by RefSeq, Jul 2008].
Function:
Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.
Subcellular Location:
Secreted.
Tissue Specificity:
Plasma.
Post-translational modifications:
Kenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
Glycated in diabetic patients.
Phosphorylation sites are present in the extracellular medium.
Acetylated on Lys-223 by acetylsalicylic acid.
DISEASE:
Familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:10720]: Form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population. Note=The disease is caused by mutations affecting the gene represented in this entry.
Similarity:
Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains.
SWISS:
P02769
Gene ID:
280717
Database links:
Entrez Gene: 100379232 Guinea pig
Entrez Gene: 100034206 Horse
Entrez Gene: 213 Human
Entrez Gene: 11657 Mouse
Entrez Gene: 396960 Pig
Entrez Gene: 100009195 Rabbit
Entrez Gene: 24186 Rat
Omim: 10720 Human
SwissProt: P49064 Cat
SwissProt: P19121 Chicken
SwissProt: P02769 Cow
SwissProt: P49822 Dog
SwissProt: P35747 Horse
SwissProt: P02768 Human
SwissProt: P07724 Mouse
SwissProt: P08835 Pig
SwissProt: P49065 Rabbit
SwissProt: P02770 Rat
Unigene: 418167 Human
Unigene: 16773 Mouse
Unigene: 202968 Rat
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Sample:
A549(Human) Cell Lysate at 30 ug
Primary: Anti-Mouse serum albumin (SL2256R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 68 kD
Observed band size: 68 kD
Sample:
Lung (Mouse) Lysate at 40 ug
Spleen (Mouse) Lysate at 40 ug
Primary: Anti-Mouse serum albumin (SL2256R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 68 kD
Observed band size: 68 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse Liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Mouse serum albumin ) Polyclonal Antibody, Unconjugated (SL2256R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Mouse serum albumin) Polyclonal Antibody, Unconjugated (SL2256R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.
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