Rabbit Anti-PD-L1 antibody | Sunlong Medical

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Programmed cell death 1 ligand 1; CD274; B7 H; B7 H1; B7 homolog 1; B7-H1; B7H; B7H1; CD 274; CD274 antigen; CD274 molecule; MGC142294; MGC142296; OTTHUMP00000021029; PD L1; PD1L1_HUMAN; PDCD1 ligand 1; PDCD1L1; PDCD1LG1; PDL 1; PDL1; Programmed death lig

Cat:

SL10159R

Species Reactivity：

Human,Mouse,Rat,(predicted: Pig,Cow,Horse,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human CD274:64-160/290<Extracellular>

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Du145(Human) Cell Lysate at 30 ugLovo(Human) Cell Lysate at 30 ugHeart(Mouse) Lysate at 40 ugMuscle(Mouse) Lysate at 40 ugHeart(Rat) Lysate at 40 ugPrimary: Anti-PD-L1 (SL10159R) at 1/500 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 50 kDObserved band size: 50 kDBlank control (blue line): Mouse spleen cells(fixed with 70% ice-cold methanol overnight at 4℃). Primary Antibody (green line): Rabbit Anti-CD274/PE-CY7 Conjugated antibody (SL10159R-PE-CY7),Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG-PE-CY7 . Blank control: Hela. Primary Antibody (green line): Rabbit Anti-PD-L1 antibody (SL10159R) Dilution: 2ug/Test; Secondary Antibody : Goat anti-rabbit IgG-FITCDilution: 0.5ug/Test. ProtocolThe cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.Blank control: Hela. Primary Antibody (green line): Rabbit Anti-PD-L1 antibody (SL10159R) Dilution: 2ug/Test; Secondary Antibody : Goat anti-rabbit IgG-FITCDilution: 0.5ug/Test. ProtocolThe cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin SLVlike and SLClike domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

Function:
Involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production.

Subunit:
Interacts with PDCD1.

Subcellular Location:
Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Endomembrane system; Single-pass type I membrane protein.

Tissue Specificity:
Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes.

Similarity:
Belongs to the immunoglobulin superfamily. BTN/MOG family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like SLVtype (immunoglobulin-like) domain.

SWISS:
Q9NZQ7

Gene ID:
29126

Database links:

Entrez Gene: 29126 Human

Entrez Gene: 60533 Mouse

Entrez Gene: 499342 Rat

Omim: 605402 Human

SwissProt: Q9NZQ7 Human

SwissProt: Q9EP73 Mouse

Unigene: 521989 Human

Unigene: 245363 Mouse

Unigene: 228198 Rat

Picture

Blank control (blue line): Mouse spleen cells(fixed with 70% ice-cold methanol overnight at 4℃).
Primary Antibody (green line): Rabbit Anti-CD274/PE-CY7 Conjugated antibody (SL10159R-PE-CY7),Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG-PE-CY7 .

Blank control: Hela.
Primary Antibody (green line): Rabbit Anti-PD-L1 antibody (SL10159R)
Dilution: 2ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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