Rabbit Anti-CYP11A1 antibody

Home > Product > Antibody > Rabbit Anti-CYP11A1 antibody

Cholesterol 20 22 desmolase； Cholesterol desmolase； Cholesterol monooxygenase (side chain cleaving)； Cholesterol side chain cleavage enzyme； Cholesterol side chain cleavage enzyme mitochondrial； Cholesterol side-chain cleavage enzyme； CP11A_HUMAN；

Cat:

SL10099R

Species Reactivity：

Human,Mouse,Rat,(predicted: Dog,Pig,Cow,Horse,Rabbit,Sheep,53/57)

Immunogen：

KLH conjugated synthetic peptide derived from human CYP11A1/P450SCC:41-140/521

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=1μg/TestICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample:Testis(Mouse)Lysate at 40 ugPrimary: Anti-CYP11A1(SL10099R)at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 53/57 kDObserved band size: 53/57 kDSample: Lane 1: Mouse Adrenal gland tissue lysates Lane 2: Rat Adrenal gland tissue lysates Primary: Anti-CYP11A1 (SL10099R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 53/57 kDaObserved band size: 47 kDaParaformaldehyde-fixed, paraffin embedded (mouse adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cytochrome P450 family 11 subfamily A polypeptide 1) Polyclonal Antibody, Unconjugated (SL10099R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cytochrome P450 family 11 subfamily A polypeptide 1) Polyclonal Antibody, Unconjugated (SL10099R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.Cell: HepG2. Concentration:1:100Host/Isotype:Rabbit/IgGFlow cytometric analysis of Rabbit IgG isotype control (Cat#: SL10099R) on HepG2(green) compared with control in the absence of primary antibody (blue) followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG(H+L) secondary antibody .

prev next

Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
Feedback Wall

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the mitochondrial inner membrane and catalyzes the conversion of cholesterol to pregnenolone, the first and rate-limiting step in the synthesis of the steroid hormones. Two transcript variants encoding different isoforms have been found for this gene. The cellular location of the smaller isoform is unclear since it lacks the mitochondrial-targeting transit peptide. [provided by RefSeq, Jul 2008]

Function:
Catalyzes the side-chain cleavage reaction of cholesterol to pregnenolone.

Subcellular Location:
Mitochondrion membrane.

DISEASE:
Defects in CYP11A1 are the cause of adrenal insufficiency congenital with 46,XY sex reversal (AICSR) [MIM:613743]. A rare disorder that can present as acute adrenal insufficiency in infancy or childhood. ACTH and plasma renin activity are elevated and adrenal steroids are inappropriately low or absent; the 46,XY patients have female external genitalia, sometimes with clitoromegaly. The phenotypic spectrum ranges from prematurity, complete underandrogenization, and severe early-onset adrenal failure to term birth with clitoromegaly and later-onset adrenal failure. Patients with congenital adrenal insufficiency do not manifest the massive adrenal enlargement typical of congenital lipoid adrenal hyperplasia.

Similarity:
Belongs to the cytochrome P450 family.

SWISS:
P05108

Gene ID:
1583

Database links:

Entrez Gene: 1583 Human

Entrez Gene: 13070 Mouse

Entrez Gene: 29136 Rat

SwissProt: P05108 Human

SwissProt: Q9QZ82 Mouse

SwissProt: P14137 Rat

Unigene: 30796 Human

Unigene: 302865 Mouse

Unigene: 1401 Rat

Picture

Sample:
Lane 1: Mouse Adrenal gland tissue lysates
Lane 2: Rat Adrenal gland tissue lysates
Primary: Anti-CYP11A1 (SL10099R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53/57 kDa
Observed band size: 47 kDa

Paraformaldehyde-fixed, paraffin embedded (mouse adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cytochrome P450 family 11 subfamily A polypeptide 1) Polyclonal Antibody, Unconjugated (SL10099R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cytochrome P450 family 11 subfamily A polypeptide 1) Polyclonal Antibody, Unconjugated (SL10099R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

Cell: HepG2.
Concentration:1:100
Host/Isotype:Rabbit/IgG
Flow cytometric analysis of Rabbit IgG isotype control (Cat#: SL10099R) on HepG2(green) compared with control in the absence of primary antibody (blue) followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG(H+L) secondary antibody .

Product Feedback Wall

Specific References (9) | SL10099R has been referenced in 9 publications.

[IF=5.201] Zhong Yuyi. et al. MIR143 Inhibits Steroidogenesis and Induces Apoptosis Repressed by H3K27me3 in Granulosa Cells. Front Cell Dev Biol. 2020 Oct;8:1159 WB ; Porcine.

PubMed:33195195

[IF=4.2] Zhang, Weidong, et al. "Decrease in male mouse fertility by hydrogen sulfide and/or ammonia can Be inheritable." Chemosphere (2017). IHSLCP ; Mouse.

PubMed:29202267

[IF=2.984] Junjie Hu. et al. Assessment of progesterone synthesis and its regulation role on dihydrotestosterone secretion in sheep epididymis. Gene. 2021 Jul;790:145699 WB,IHC ; Sheep.

PubMed:3396876

[IF=10.588] Tianxin Zhao. et al. Prenatal exposure to environmentally relevant levels of PBDE-99 leads to testicular dysgenesis with steroidogenesis disorders. J Hazard Mater. 2022 Feb;424:127547 IHC ; Mouse.

PubMed:10.1016/j.jhazmat.2021.127547

[IF=1.537] R. Fan . et al. Effects of the FABP4 gene on steroid hormone secretion in goose ovarian granulosa cells. Brit Poultry Sci. 2021;62(1):81-91 WB ; Goose.

PubMed:32875818

[IF=3.813] Wenqian Xie. et al. Seasonal expressions of SF-1, StAR and P450scc in the scent glands of the muskrats (Ondatra zibethicus). J Steroid Biochem. 2020 Nov;204:105766 IHC ; Muskrat.

PubMed:32991988

[IF=2.094] Xiaodan Li. et al. Effects of SPATA6 on proliferation, apoptosis and steroidogenesis of Hu sheep Leydig cells in vitro. Theriogenology. 2021 May;166:9 WB ; Sheep.

PubMed:33667862

[IF=2.638] An SY et al. MiR-1197-3p regulates testosterone secretion in goat Leydig cells via targeting PPARGC1A. Gene. 2019 May 31;710:131-139. WB ; Goat.

PubMed:31158446

[IF=4.522] Xiao L et al. Dihydrotestosterone synthesis in the sheep corpus luteum and its potential mechanism in luteal regression. J Cell Physiol. 2019 Jan 22. WB ; Sheep.

PubMed:30671954