Rabbit Anti-B7-H6 antibody | Sunlong Medical

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B7 homolog 6; B7-H6; B7H6; B7H6_HUMAN; DKFZp686O24166; Natural cytotoxicity triggering receptor 3 ligand 1; NCR3LG1; Putative Ig like domain containing protein DKFZp686O24166/DKFZp686I21167.

Cat:

SL12559R

Species Reactivity：

Human,Mouse,

Immunogen：

KLH conjugated synthetic peptide derived from human B7-H6:51-150/454<Extracellular>

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Heart (Mouse) Lysate at 40 ugLiver (Mouse) Lysate at 40 ugPrimary: Anti- B7-H6 (SL12559R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 48 kDObserved band size: 50 kDSample: Huh7 Cell (Human) Lysate at 40 ugA549 Cell (Human) Lysate at 40 ugPrimary: Anti-B7-H6 (SL12559R) at 1/300 dilutionSecondary: HRP conjugated Goat-Anti-rabbit IgG (SL0295G-HRP) at 1/5000 dilutionPredicted band size: 48 kDObserved band size: 48 kDSample: Cerebral cortex (Mouse) Lysate at 40 ugHippocampus (Mouse) Lysate at 40 ugPrimary: Anti- B7-H6 (SL12559R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 48 kDObserved band size: 50 kD Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (B7-H6) Polyclonal Antibody, Unconjugated (SL12559R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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B7H6 belongs to the B7 family (see MIM 605402) and is selectively expressed on tumor cells. Interaction of B7H6 with NKp30 (NCR3; MIM 611550) results in natural killer (NK) cell activation and cytotoxicity (Brandt et al., 2009 [PubMed 19528259]).[supplied by OMIM, Jan 2011].

Function:
Triggers NCR3-dependent natural killer cell activation.

Subunit:
Monomer. Interacts specifically with NCR3, but not with other natural killer cell-activating receptors, including NCR1, NCR2 and KLRK1.

Subcellular Location:
Cell membrane; Single-pass type I membrane protein.

Tissue Specificity:
Not detected in any normal tissue tested. Expressed at the surface of several tumor cell lines including T and B lymphomas, myeloid leukemias, melanomas, carcinomas and large T SV40 antigen-transformed cells (at protein level).

Similarity:
Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
Contains 1 Ig-like SLVtype (immunoglobulin-like) domain.

SWISS:
Q68D85

Gene ID:
374383

Database links:

Entrez Gene: 374383 Human

Omim: 613714 Human

SwissProt: Q68D85 Human

Unigene: 146274 Human

Picture

Sample:
Huh7 Cell (Human) Lysate at 40 ug
A549 Cell (Human) Lysate at 40 ug
Primary: Anti-B7-H6 (SL12559R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (SL0295G-HRP) at 1/5000 dilution
Predicted band size: 48 kD
Observed band size: 48 kD

Sample:
Cerebral cortex (Mouse) Lysate at 40 ug
Hippocampus (Mouse) Lysate at 40 ug
Primary: Anti- B7-H6 (SL12559R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 50 kD

Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (B7-H6) Polyclonal Antibody, Unconjugated (SL12559R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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