Rabbit Anti-Phospho-c-Fos (Ser374) antibody

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c-Fos (phospho S374); c-Fos (phospho-Ser374); c-Fos (phospho Ser374); p-c-Fos (Ser374); Cellular oncogene fos; FBJ murine osteosarcoma viral v fos oncogene homolog antibody FBJ Osteosarcoma Virus; FOS; FOS protein; G0 G1 switch regulatory protein 7; G0S7

Cat:

SL12911R

Species Reactivity：

Mouse,Rat,(predicted: Human,)

Immunogen：

KLH conjugated Synthesised phosphopeptide derived from human c-Fos around the phosphorylation site of Ser374:LS(p-S)PT

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Cerebrum (Mouse) Lysate at 40 ugCerebrum (Rat) Lysate at 40 ugPrimary: Anti-Phospho-c-Fos (Ser374) (SL12911R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 62/46 kDObserved band size: 46 kDSample: NIH/3T3 Cell (Mouse) Lysate at 30 ugPrimary: Anti- Phospho-c-Fos (Ser374) (SL12911R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 41 kDObserved band size: 36 kDSample: Jurkat Cell Lysate at 30 ugPrimary: Anti- Phospho-c-Fos (Ser374) (SL12911R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 41 kDObserved band size: 36 kD Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-c-Fos (Ser374)) Polyclonal Antibody, Unconjugated (SL12911R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-c-Fos (Ser374)) Polyclonal Antibody, Unconjugated (SL12911R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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Unit:

Price: $256.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death. [provided by RefSeq, Jul 2008].

Function:
Nuclear phosphoprotein which forms a tight butnon-covalently linked complex with the JUN/AP-1 transcriptionfactor. In the heterodimer, FOS and JUN/AP-1 basic regions eachseems to interact with symmetrical DNA half sites. On TGF-betaactivation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at theAP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Hasa critical function in regulating the Has a critical function inregulating the development of cells destined to form and maintainthe skeleton. It is thought to have an important role in signaltransduction, cell proliferation and differentiation.

Subunit:
Heterodimer; with JUN (By similarity). Interacts withMAFB. Component of the SMAD3/SMAD4/JUN/FOS complexrequired for syngernistic TGF-beta-mediated transcription at theAP1 promoter site. Interacts with SMAD3; the interaction is weakeven on TGF-beta activation. Interacts with MAFB. Interacts withDSIPI; this interaction inhibits the binding of active AP1 to itstarget DNA.

Subcellular Location:
Nucleus.

Post-translational modifications:
Phosphorylated in the SLCterminal upon stimulation by nerve growth factor (NGF) and epidermal growth factor (EGF). Phosphorylated, in vitro, by MAPK and RSK1. Phosphorylation on both Ser-362 and Ser-374 by MAPK1/2 and RSK1/2 leads to protein stabilization with phosphorylation on Ser-374 being the major site for protein stabilization on NGF stimulation. Phosphorylation on Ser-362 and Ser-374 primes further phosphorylations on Thr-325 and Thr-331 through promoting docking of MAPK to the DEF domain. Phosphorylation on Thr-232, induced by HA-RAS, activates the transcriptional activity and antagonizes sumoylation. Phosphorylation on Ser-362 by RSK2 in osteoblasts contributes to osteoblast transformation. Constitutively sumoylated by SUMO1, SUMO2 and SUMO3. Desumoylated by SENP2. Sumoylation requires heterodimerization with JUN and is enhanced by mitogen stimulation. Sumoylation inhibits the AP-1 transcriptional activity and is, itself, inhibited by Ras-activated phosphorylation on Thr-232.

Similarity:
Belongs to the bZIP family. Fos subfamily.
Contains 1 bZIP domain.

SWISS:
P01100

Gene ID:
2353

Database links:

Entrez Gene: 2353 Human

Entrez Gene: 14281 Mouse

Entrez Gene: 314322 Rat

Omim: 164810 Human

SwissProt: P01100 Human

SwissProt: P01101 Mouse

SwissProt: P12841 Rat

Unigene: 246513 Mouse

Unigene: 103750 Rat

Picture

Sample:
NIH/3T3 Cell (Mouse) Lysate at 30 ug
Primary: Anti- Phospho-c-Fos (Ser374) (SL12911R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 36 kD

Sample:
Jurkat Cell Lysate at 30 ug
Primary: Anti- Phospho-c-Fos (Ser374) (SL12911R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 36 kD

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-c-Fos (Ser374)) Polyclonal Antibody, Unconjugated (SL12911R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-c-Fos (Ser374)) Polyclonal Antibody, Unconjugated (SL12911R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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