Rabbit Anti-S-tag antibody | Sunlong Medical

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Stag; S tag; Ribonuclease A; KETAAAKFERQHMDS.

Cat:

SL33017R

Species Reactivity：

Recombinant protein

Immunogen：

KLH conjugated synthetic peptide S-tag

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:1000-5000ELISA=1:5000-10000not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Lane1: S-Tagged Fusion Protein Overexpression E.coli Lysate at 2ug Lane2: S-Tagged Fusion Protein Overexpression E.coli Lysate at 2ug Primary: Anti-S-tag (SL33017R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 41 kD Observed band size: 45 kD

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Unit:

Price: $196.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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S-tag is the name of an oligopeptide derived from pancreatic ribonuclease A (RNase A). If RNase A is digested with subtilisin, a single peptide bond is cleaved, but the resulting two products remain weakly bound to each other and the protein, called ribonuclease S, remains active although each of the two products alone shows no enzymatic activity. The N-terminus of the original RNase A, also called S-peptide, consists of 20 amino acid residues, of which only the first 15 are required for ribonuclease activity. This 15 amino acids long peptide is called S15 or S-tag.
The amino acid sequence of the S-tag is: Lys-Glu-Thr-Ala-Ala-Ala-Lys-Phe-Glu-Arg-Gln-His-Met-Asp-Ser. It is believed that the peptide with its abundance of charged and polar residues could improve solubility of proteins it is attached to[citation needed]. Moreover, the peptide alone is thought not to fold into a distinct structure. On DNA-level the S-tag can be attached to the N- or SLCterminus of any protein. After gene expression, such a tagged protein can be detected by commercially available antibodies.

SWISS:
N/A

Gene ID:
N/A

Picture

Sample:
Lane1: S-Tagged Fusion Protein Overexpression E.coli Lysate at 2ug
Lane2: S-Tagged Fusion Protein Overexpression E.coli Lysate at 2ug
Primary: Anti-S-tag (SL33017R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 45 kD

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