Peroxidase (POD, EC 1.11.1.7) widely exists in animals, plants and microorganisms. It can catalyze the
oxidation of phenols and amines by hydrogen peroxide, and has the dual effect of eliminating toxicity of
hydrogen peroxide, phenols and amines. In the presence of hydrogen peroxide, POD can catalyzes H2O2
oxidize specific substrates to produce one substance which has a absorption at 470 nm.
Reagents and Equipment Required but Not Provided:
Spectrophotometer, desk centrifuge, transferpettor, 1 mL glass cuvette, mortar/homogeniser, ice and
distilled water.